About: PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans

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About: PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and quantitative PCR method we revealed possible changes originating in the overall bacterial composition and also in the subpopulation of Bifidobacterium group. DGGE profiles displayed high complexity and individuality for each subject. Considerable variations in the composition of band patterns were observed among different persons. A raised level of fecal Bacteroides in response to chitosan intake was found in all samples. Bifidobacterium levels following chitosan intake increased or remain unchanged. Non-significant increase was, surprisingly, found in the numbers of butyrate-producing bacteria. A feeding study was performed to monitor the effect of chitosan intake on the fecal microbiota of ten healthy human subjects. Diversity of microflora was monitored during 8 weeks including 4 weeks of chitosan supplementations. Using denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene amplicons and quantitative PCR method we revealed possible changes originating in the overall bacterial composition and also in the subpopulation of Bifidobacterium group. DGGE profiles displayed high complexity and individuality for each subject. Considerable variations in the composition of band patterns were observed among different persons. A raised level of fecal Bacteroides in response to chitosan intake was found in all samples. Bifidobacterium levels following chitosan intake increased or remain unchanged. Non-significant increase was, surprisingly, found in the numbers of butyrate-producing bacteria. (en)
Title	PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans (en)
skos:prefLabel	PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans (en)
skos:notation	RIV/67985904:_____/10:00345859!RIV11-GA0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA525/08/0803), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Mrázek, Jakub Kopečný, Jan Koppová, Ingrid Šimůnek, Jiří Fliegerová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	278338
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/10:00345859
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Chitosan; Microbial stability; Microflora (en)
http://linked.open.../riv/klicoveSlovo	Chitosan Microflora Microbial stability
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[BFC2D1CF5D03]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	FB
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	The effect of chitooligosaccharides in the human bowel
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Fliegerová, Kateřina Kopečný, Jan Koppová, Ingrid Mrázek, Jakub Šimůnek, Jiří
http://linked.open...ain/vavai/riv/wos	000281277900012
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0015-5632
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans PCR-DGGE-Based Study of Fecal Microbial Stability during the Long-Term Chitosan Supplementation of Humans

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