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  • The multiprotein complexes known as condensins (I and II) are major players in chromosome dynamics in mitotic and meiotic cells. Here, we report for the first time the detection of different condensin subunits from both complexes in mammalian oocytes. Using immunoblotting analysis we examined expression levels of condensin subunits during meiotic maturation of porcine oocytes. The expression of the core subunit structural maintenance of chromosomes 2 (SMC2), identical in both condensin complexes, did not change significantly during maturation. Similarly, there was no significant change in the expression of the chromosome associated protein (CAP)-H and CAP-H2 subunits, components of condensin I and II, respectively. Conversely, the expression profiles of CAP-G, CAP-D2 (condensin I) and CAP-D3 (condensin II) were more interesting. At least two isoforms of the CAP-D2 subunit were detected, along with three isoforms of the CAP-D3 and CAP-G subunits.
  • The multiprotein complexes known as condensins (I and II) are major players in chromosome dynamics in mitotic and meiotic cells. Here, we report for the first time the detection of different condensin subunits from both complexes in mammalian oocytes. Using immunoblotting analysis we examined expression levels of condensin subunits during meiotic maturation of porcine oocytes. The expression of the core subunit structural maintenance of chromosomes 2 (SMC2), identical in both condensin complexes, did not change significantly during maturation. Similarly, there was no significant change in the expression of the chromosome associated protein (CAP)-H and CAP-H2 subunits, components of condensin I and II, respectively. Conversely, the expression profiles of CAP-G, CAP-D2 (condensin I) and CAP-D3 (condensin II) were more interesting. At least two isoforms of the CAP-D2 subunit were detected, along with three isoforms of the CAP-D3 and CAP-G subunits. (en)
Title
  • Detection of condensin I and II in maturing pig oocytes
  • Detection of condensin I and II in maturing pig oocytes (en)
skos:prefLabel
  • Detection of condensin I and II in maturing pig oocytes
  • Detection of condensin I and II in maturing pig oocytes (en)
skos:notation
  • RIV/67985904:_____/10:00341856!RIV11-GA0-67985904
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA204/06/1297), P(GD204/09/H084), P(GP524/09/P435), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 253635
http://linked.open...ai/riv/idVysledku
  • RIV/67985904:_____/10:00341856
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Chromosome condensation; Female meiosis; Phosphorylation (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • AU - Australské společenství
http://linked.open...ontrolniKodProRIV
  • [D87BFF95A1FD]
http://linked.open...i/riv/nazevZdroje
  • Reproduction Fertility and Development
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 22
http://linked.open...iv/tvurceVysledku
  • Kubelka, Michal
  • Karabínová, Pavla
  • Šušor, Andrej
  • Šašková, Adéla
  • Lišková, Lucie
  • Pivoňková, Kateřina
http://linked.open...ain/vavai/riv/wos
  • 000276106000007
http://linked.open...n/vavai/riv/zamer
issn
  • 1031-3613
number of pages
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