About: Gene expression during minor genome activation in preimplantation bovine development

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The main goal of this study was to identify mRNA transcripts, whose content increases during bovine minor embryonic genome activation. We compared the gene expression profile of the bovine 4-cell stage embryo and MII oocyte using the technique of suppression subtractive hybridization. Differentially expressed amplicons were subcloned and 60 of them were sequenced. The resulting DNA sequences were compared with GenBank databases using Blast search. The expression of five differentially expressed genes with an apparent function in cell cycle progression, chromatin remodeling, and splicing or translation initiation was further characterized by a real-time RT-PCR. Splicing factor, arginine/serine-rich 3 (SRFS3) represents the first described gene with an important function in pre-implantation development, which is also expressed during bovine minor genome activation and it is alpha-amanitin sensitive during this period. All described genes can play an important role in the pre-implantation development ... The main goal of this study was to identify mRNA transcripts, whose content increases during bovine minor embryonic genome activation. We compared the gene expression profile of the bovine 4-cell stage embryo and MII oocyte using the technique of suppression subtractive hybridization. Differentially expressed amplicons were subcloned and 60 of them were sequenced. The resulting DNA sequences were compared with GenBank databases using Blast search. The expression of five differentially expressed genes with an apparent function in cell cycle progression, chromatin remodeling, and splicing or translation initiation was further characterized by a real-time RT-PCR. Splicing factor, arginine/serine-rich 3 (SRFS3) represents the first described gene with an important function in pre-implantation development, which is also expressed during bovine minor genome activation and it is alpha-amanitin sensitive during this period. All described genes can play an important role in the pre-implantation development ... (en)
Title	Gene expression during minor genome activation in preimplantation bovine development Gene expression during minor genome activation in preimplantation bovine development (en)
skos:prefLabel	Gene expression during minor genome activation in preimplantation bovine development Gene expression during minor genome activation in preimplantation bovine development (en)
skos:notation	RIV/67985904:_____/09:00328317!RIV10-AV0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA523/06/1226), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Němcová, Lucie Kaňka, Jiří Vodičková Kepková, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	316050
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/09:00328317
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	developmental biology; embryo; gene expression; real-time RT-PCR (en)
http://linked.open.../riv/klicoveSlovo	gene expression embryo developmental biology real-time RT-PCR
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C8DB90810D6E]
http://linked.open...i/riv/nazevZdroje	Theriogenelogy
http://linked.open...in/vavai/riv/obor	GI
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Embryonics of pre-implantation mammalian development
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	72
http://linked.open...iv/tvurceVysledku	Němcová, Lucie Kaňka, Jiří Kepková, Kateřina
http://linked.open...ain/vavai/riv/wos	000268901700019
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0093-691X
number of pages	12 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Gene expression during minor genome activation in preimplantation bovine development Gene expression during minor genome activation in preimplantation bovine development Gene expression during minor genome activation in preimplantation bovine development Gene expression during minor genome activation in preimplantation bovine development

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