About: Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We show in the present study that freshly isolated pig cumulus-oocyte complexes (COCs) display a limited response to LH, as assessed by the expression of hyaluronan synthase 2 (Has2) mRNA, activation of protein kinase A (PKA), production of hyaluronic acid (HA) and progesterone, cumulus cell expansion and resumption of meiosis. These data indicate that freshly isolated COCs do not possess a sufficient number of functional LH receptors (LHR). However, the expression of Lhr significantly increased during the culture of COCs in vitro in a medium supplemented with FSH. Assuming that the effect of FSH on LHR induction is mediated via cAMP signaling pathways, we developed a new culture system, in which the COCs were pre-cultured for 72 h in a medium supplemented with dbcAMP. The pre-cultured COCs remained in the germinal vesicle stage, their cumulus investment underwent a dramatic increase in size and gap junctions between the cumulus cells were preserved. The stimulation of such COCs with either FSH or ... We show in the present study that freshly isolated pig cumulus-oocyte complexes (COCs) display a limited response to LH, as assessed by the expression of hyaluronan synthase 2 (Has2) mRNA, activation of protein kinase A (PKA), production of hyaluronic acid (HA) and progesterone, cumulus cell expansion and resumption of meiosis. These data indicate that freshly isolated COCs do not possess a sufficient number of functional LH receptors (LHR). However, the expression of Lhr significantly increased during the culture of COCs in vitro in a medium supplemented with FSH. Assuming that the effect of FSH on LHR induction is mediated via cAMP signaling pathways, we developed a new culture system, in which the COCs were pre-cultured for 72 h in a medium supplemented with dbcAMP. The pre-cultured COCs remained in the germinal vesicle stage, their cumulus investment underwent a dramatic increase in size and gap junctions between the cumulus cells were preserved. The stimulation of such COCs with either FSH or ... (en)
Title	Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system (en)
skos:prefLabel	Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system (en)
skos:notation	RIV/67985904:_____/09:00326146!RIV10-MZE-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA523/08/0111), P(QG50052), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Nagyová, Eva Němcová, Lucie Procházka, Radek
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	310056
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/09:00326146
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	LH Receptors; oocyte (en)
http://linked.open.../riv/klicoveSlovo	LH Receptors oocyte
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[14FC0A204E54]
http://linked.open...i/riv/nazevZdroje	Molecular Reproduction and Development
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Potential of EGF-like peptides and synthetic analogues of cAMP to enhance developmental capacity of pig oocytes cultured in vitro Reproductive biotechniques in pigs
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	76
http://linked.open...iv/tvurceVysledku	Mlynarčíková, A. Nagyová, Eva Němcová, Lucie Procházka, Radek Scsuková, S.
http://linked.open...ain/vavai/riv/wos	00026800640000
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	1040-452X
number of pages	11 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system Development of functional LH Receptors on pig cumulus-oocyte complexes cultured in vitro by a novel two-step culture system

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