About: Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Chitinolytické aktivity Clostridia sp. JM2 izolovaného z lidské stolice nahrazeného chitosanem. (cs) The novel chitinolytic bacterium Clostridium beijerinckii strain JM2 was isolated from the stool of healthy volunteers supplied daily per orally with 3 g of chitosan. The bacterium grown on colloidal chitin produced a complete array of chitinolytic enzymes. Significant activities of endochitinase, exochitinase and chitosanase were excreted into the medium (301, 282 and 268 nkat/mu g protein, respectively). The high cellular activity of N-acetyl-beta-glucosaminidase (NAGase) and chitosanase were detected (732.4 and 154 nkat/mu g protein, respectively). NAGase activity represented the main activity associated with the cellular fraction. The activities of both enzymes tested increased from 20 to 50 C; the optimum reaction temperature estimated being 50 C. Endochitinase as well as NAGase showed an activity in the pH interval of 4.0-8.0; the optimum pH values were 6.5 and 6.0, respectively. The novel chitinolytic bacterium Clostridium beijerinckii strain JM2 was isolated from the stool of healthy volunteers supplied daily per orally with 3 g of chitosan. The bacterium grown on colloidal chitin produced a complete array of chitinolytic enzymes. Significant activities of endochitinase, exochitinase and chitosanase were excreted into the medium (301, 282 and 268 nkat/mu g protein, respectively). The high cellular activity of N-acetyl-beta-glucosaminidase (NAGase) and chitosanase were detected (732.4 and 154 nkat/mu g protein, respectively). NAGase activity represented the main activity associated with the cellular fraction. The activities of both enzymes tested increased from 20 to 50 C; the optimum reaction temperature estimated being 50 C. Endochitinase as well as NAGase showed an activity in the pH interval of 4.0-8.0; the optimum pH values were 6.5 and 6.0, respectively. (en)
Title	Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan Chitinolytické aktivity Clostridia sp. JM2 izolovaného z lidské stolice nahrazeného chitosanem (cs) Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan (en)
skos:prefLabel	Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan Chitinolytické aktivity Clostridia sp. JM2 izolovaného z lidské stolice nahrazeného chitosanem (cs) Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan (en)
skos:notation	RIV/67985904:_____/08:00325027!RIV09-AV0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA525/05/2584), Z(AV0Z40500505), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Koppová, Ingrid Šimůnek, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	359794
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/08:00325027
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Clostridium; chitosan (en)
http://linked.open.../riv/klicoveSlovo	Clostridium chitosan
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[3734F9E8454E]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	The effect of chitin and chitosan in the human bowel
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Koppová, Ingrid Tishchenko, Galina Šimůnek, Jiří
http://linked.open...ain/vavai/riv/wos	000257941200016
http://linked.open...n/vavai/riv/zamer	Progresivní makromolekulární materiály a supramolekulární systémy: syntéza a studium vlastností, jevů a možností využití pro speciální aplikace a moderní technologie Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0015-5632
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered per orally by chitosan

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