About: Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The Vibrio harveyi BB170 autoinducer bioassay was used to detect possible quorum sensing autoinducer 2 molecule (AI-2) in culture fluids of commensal intestinal bacteria. The assay showed that the culture fluids of Bacteroides vulgatus, Clostridium proteoclasticum, Escherichia coli, Eubacterium rectale, Lachnospira multipara, Pseudobutyrivibrio ruminis, Roseburia intestinalis, Ruminococcus albus and Ruminococcus flavefaciens contained AI-2-like molecules. Furthermore, we could also amplify bands from some of the tested strains using primers designed to the luxS gene. These findings suggest that the AI-2 molecule is present in the gastrointestinal tract, but it was not proved up to now that is used for bacterial cell-to-cell communication. The Vibrio harveyi BB170 autoinducer bioassay was used to detect possible quorum sensing autoinducer 2 molecule (AI-2) in culture fluids of commensal intestinal bacteria. The assay showed that the culture fluids of Bacteroides vulgatus, Clostridium proteoclasticum, Escherichia coli, Eubacterium rectale, Lachnospira multipara, Pseudobutyrivibrio ruminis, Roseburia intestinalis, Ruminococcus albus and Ruminococcus flavefaciens contained AI-2-like molecules. Furthermore, we could also amplify bands from some of the tested strains using primers designed to the luxS gene. These findings suggest that the AI-2 molecule is present in the gastrointestinal tract, but it was not proved up to now that is used for bacterial cell-to-cell communication. (en) U komensálních střevních bakterií byla pomocí autoindukčního systému Vibrio harveyi BB170 testována přítomnost molekul AI-2 systému pro quorum sensing. V kulturách Bacteroides vulgatus, Clostridium proteoclasticum, Escherichia coli, Eubacterium rectale, Lachnospira multipara, Pseudobutyrivibrio ruminis, Roseburia intestinalis, Ruminococcus albus a Ruminococcus flavefaciens byla prokázána přítomnost látek podobných AI-2. Navíc se podařilo pomocí primerů specifických pro luxS gen prokázat přítomnost tohoto genu ve většině testovaných kultur. To znamená, že součásti systému quorum sensing jsou u nich přítomny, pouze se zatím neprokázalo, že slouží ke komunikaci mezi buňkami. (cs)
Title	Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria Důkaz přítomnosti článků AI-2 quorum sensing systému u střevních bakterií lidí (cs) Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria (en)
skos:prefLabel	Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria Důkaz přítomnosti článků AI-2 quorum sensing systému u střevních bakterií lidí (cs) Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria (en)
skos:notation	RIV/67985904:_____/08:00309954!RIV09-AV0-67985904
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA303/06/0974), Z(AV0Z50450515)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Lukáš, Filip Kopečný, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav živočišné fyziologie a genetiky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	362833
http://linked.open...ai/riv/idVysledku	RIV/67985904:_____/08:00309954
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	quorum sensing system (en)
http://linked.open.../riv/klicoveSlovo	quorum sensing system
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[E5B91B700C0F]
http://linked.open...i/riv/nazevZdroje	Folia Microbiologica
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Interaction of commensal and probiotic bacteria with gut mucosa studied in gnotobiotic models
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	53
http://linked.open...iv/tvurceVysledku	Kopečný, Jan Lukáš, Filip Gorenc, G.
http://linked.open...ain/vavai/riv/wos	000257941200009
http://linked.open...n/vavai/riv/zamer	Genetic, functional and developmental potential of animal cells, tissues and organisms: their use in medicine, ecology and agriculture
issn	0015-5632
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria Detection of Possible AI-2-mediated Quorum Sensing System in Commensal Intestinal Bacteria

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