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Description
  • We present a portable surface plasmon resonance (SPR) sensor based on spectroscopy of surface plasmons on a special diffractive structure. The sensor combines a microfluidic cartridge incorporating the special diffractive structure functionalized with DNA probes and a compact SPR reader. We apply the sensor to detection of nucleic acids employing two most common immobilization methods: (a) biotinylated probes immobilized using the biotin-streptavidin chemistry and (b) thiolated probes attached directly to the surface of the SPR sensor. It is demonstrated that both these immobilization methods allow detecting short nucleic acids at levels below 100 pM
  • We present a portable surface plasmon resonance (SPR) sensor based on spectroscopy of surface plasmons on a special diffractive structure. The sensor combines a microfluidic cartridge incorporating the special diffractive structure functionalized with DNA probes and a compact SPR reader. We apply the sensor to detection of nucleic acids employing two most common immobilization methods: (a) biotinylated probes immobilized using the biotin-streptavidin chemistry and (b) thiolated probes attached directly to the surface of the SPR sensor. It is demonstrated that both these immobilization methods allow detecting short nucleic acids at levels below 100 pM (en)
Title
  • Portable surface plasmon resonance biosensor for detection of nucleic acids
  • Portable surface plasmon resonance biosensor for detection of nucleic acids (en)
skos:prefLabel
  • Portable surface plasmon resonance biosensor for detection of nucleic acids
  • Portable surface plasmon resonance biosensor for detection of nucleic acids (en)
skos:notation
  • RIV/67985882:_____/11:00437420!RIV15-AV0-67985882
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z20670512)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 221562
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  • RIV/67985882:_____/11:00437420
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  • Surface plasmon resonance; Portable biosensor; Oligonucleotides (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [1C9825D6D275]
http://linked.open...v/mistoKonaniAkce
  • Athens
http://linked.open...i/riv/mistoVydani
  • Amsterdam
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  • Procedia Engineering
http://linked.open...in/vavai/riv/obor
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  • Adam, Pavel
  • Homola, Jiří
  • Piliarik, Marek
  • Šípová, Hana
  • Hegnerová, Kateřina
  • Vala, Milan
  • Bocková, Markéta
  • Chadt, Karel
http://linked.open...vavai/riv/typAkce
http://linked.open...ain/vavai/riv/wos
  • 000300512400035
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
issn
  • 1877-7058
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.proeng.2011.12.037
http://purl.org/ne...btex#hasPublisher
  • Elsevier
https://schema.org/isbn
  • 978-1-62748-581-4
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