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  • Local chemical sensing in living cells by fluorescence methods with submicron spatial resolution is in the scope of biologist because of bringing new information about biochemical processes in living matter [1]. One of the most important monitored variables is pH. Despite of progress of novel submicron probes suitable for in-situ measurement in living cells [1] and biological micro samples [2] still there is a lack off suitable opto-chemical transducers sensitive around pH 5-7 limiting development of novel fluorescence sensors. Moreover, the interaction of the immobilized transducer with the matrix can strongly affect its fluorescence properties.
  • Local chemical sensing in living cells by fluorescence methods with submicron spatial resolution is in the scope of biologist because of bringing new information about biochemical processes in living matter [1]. One of the most important monitored variables is pH. Despite of progress of novel submicron probes suitable for in-situ measurement in living cells [1] and biological micro samples [2] still there is a lack off suitable opto-chemical transducers sensitive around pH 5-7 limiting development of novel fluorescence sensors. Moreover, the interaction of the immobilized transducer with the matrix can strongly affect its fluorescence properties. (en)
Title
  • Preparation and characterization of sensing layers for pH detection in living plant cells
  • Preparation and characterization of sensing layers for pH detection in living plant cells (en)
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  • Preparation and characterization of sensing layers for pH detection in living plant cells
  • Preparation and characterization of sensing layers for pH detection in living plant cells (en)
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  • RIV/67985882:_____/11:00374704!RIV12-AV0-67985882
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  • Z(AV0Z20670512)
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  • 223045
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  • RIV/67985882:_____/11:00374704
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  • surface effects; fluorescence; photonic crystal fibers (en)
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  • [5C2FA41FD04E]
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  • Praha
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  • Bellingham
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  • Optical Sensors 2011 and Photonic Crystal Fibers V (Proceedings of SPIE Vol. 8073)
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  • Kašík, Ivan
  • Martan, Tomáš
  • Matějec, Vlastimil
  • Mrázek, Jan
  • Podrazký, Ondřej
  • Pospíšilová, Marie
  • Nekola, J.
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  • 000293740400030
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  • SPIE
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  • 978-0-8194-8663-9
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