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  • We report a novel high-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of protein biomarkers. The biosensor is based on a high-performance SPR imaging sensor with polarization contrast and internal referencing which yields a considerably higher sensitivity and resolution than conventional SPR imaging systems (refractive index resolution 2 10-7 RIU). We combined the SPR imaging biosensor with microspotting to create an array of antibodies. DNA-directed protein immobilization was utilized for the spatially resolved attachment of antibodies. Using Human Chorionic Gonadotropin (hCG) as model protein biomarker, we demonstrated the potential for simultaneous detection of proteins in up to 100 channels.
  • We report a novel high-throughput surface plasmon resonance (SPR) biosensor for rapid and parallelized detection of protein biomarkers. The biosensor is based on a high-performance SPR imaging sensor with polarization contrast and internal referencing which yields a considerably higher sensitivity and resolution than conventional SPR imaging systems (refractive index resolution 2 10-7 RIU). We combined the SPR imaging biosensor with microspotting to create an array of antibodies. DNA-directed protein immobilization was utilized for the spatially resolved attachment of antibodies. Using Human Chorionic Gonadotropin (hCG) as model protein biomarker, we demonstrated the potential for simultaneous detection of proteins in up to 100 channels. (en)
Title
  • Surface plasmon resonance imaging for parallelized detection of protein biomarkers
  • Surface plasmon resonance imaging for parallelized detection of protein biomarkers (en)
skos:prefLabel
  • Surface plasmon resonance imaging for parallelized detection of protein biomarkers
  • Surface plasmon resonance imaging for parallelized detection of protein biomarkers (en)
skos:notation
  • RIV/67985882:_____/09:00345547!RIV12-AV0-67985882
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(KAN200670701), Z(AV0Z20670512)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 344778
http://linked.open...ai/riv/idVysledku
  • RIV/67985882:_____/09:00345547
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Surface plasmon resonance imaging; Biosensor; Protein detection (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [E250341B84AE]
http://linked.open...v/mistoKonaniAkce
  • Praha
http://linked.open...i/riv/mistoVydani
  • Bellingham, Washington
http://linked.open...i/riv/nazevZdroje
  • Optical Sensors 2009
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Homola, Jiří
  • Piliarik, Marek
  • Vaisocherová, Hana
  • Párová, Lucie
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://bibframe.org/vocab/doi
  • 10.1117/12.820882
http://purl.org/ne...btex#hasPublisher
  • SPIE
https://schema.org/isbn
  • 9780819476302
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