About: Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	V této práci byla metoda SPR porovnána s metodou ELISA pro přímou a amplifikovanou detekci perspektivního biomárkru rakoviny – CD166 / ALCAM v pufru a ve zředěném lidském krevním séru pacientů s rakovinou a zdravými jedinci. Je ukázáno mmo jiné, že metoda SPR může rozlišit hladiny ALCAMu mezí pozitivními a negativními vzorky séra přímou detekcí bez nutnosti amplifikace. (cs) In this work, an ptimized SPR assay was implemented and its performance compared with an ELISA assay for CD166/activated cell leukocyte adhesion molecule (ALCAM), as candidate pancreatic cancer marker, based on direct and amplified detection in buffer and in human serum samples from healthy individuals and subjects with cancer. It is demonstrated that SPR can distinguish between ALCAM levels in cancer and control sera using direct detection without the need for additional amplification steps. In this work, an ptimized SPR assay was implemented and its performance compared with an ELISA assay for CD166/activated cell leukocyte adhesion molecule (ALCAM), as candidate pancreatic cancer marker, based on direct and amplified detection in buffer and in human serum samples from healthy individuals and subjects with cancer. It is demonstrated that SPR can distinguish between ALCAM levels in cancer and control sera using direct detection without the need for additional amplification steps. (en)
Title	Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera Srovnávací studie metod SPR a ELISA založená na analýze vzorků krevního séra pacientů s rakovinou a zdravých jedinců na přítomnost CD166/ALCAM (cs) Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera (en)
skos:prefLabel	Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera Srovnávací studie metod SPR a ELISA založená na analýze vzorků krevního séra pacientů s rakovinou a zdravých jedinců na přítomnost CD166/ALCAM (cs) Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera (en)
skos:notation	RIV/67985882:_____/09:00326027!RIV09-AV0-67985882
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z20670512)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Vaisocherová, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fotoniky a elektroniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	307652
http://linked.open...ai/riv/idVysledku	RIV/67985882:_____/09:00326027
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	SPR; ELISA; Cancer biomarkers (en)
http://linked.open.../riv/klicoveSlovo	Cancer biomarkers ELISA SPR
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[95EEC17CDEAC]
http://linked.open...i/riv/nazevZdroje	Biosensors and Bioeletronics
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	24
http://linked.open...iv/tvurceVysledku	Jiang, S. Taylor, A. D. Vaisocherová, Hana Faca, V. M. Hanash, S.
http://linked.open...n/vavai/riv/zamer	Materiály, struktury, systémy a signály v elektronice, optoelektronice a fotonice
issn	0956-5663
number of pages	6 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera Comparative study of SPR and ELISA methods based on analysis of CD166/ALCAM levels in cancer and control human sera

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