About: Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance

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About: Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We report a surface plasmon resonance (SPR) sensor for detection of thrombin via DNA aptamers immobilized on the surface. An effect of the immobilisation method is investigated. Immobilization methods included: (i) chemisorption of aptamers modified with SH groups, (ii) immobilisation of biotin-tagged aptamers via previously immobilized avidin, neutravidin or streptavidin, (iii) aptamer-dendrimer method. Immobilisation of aptamers by the streptavidin-biotin yields the best sensor specificity and sensitivity. We report a surface plasmon resonance (SPR) sensor for detection of thrombin via DNA aptamers immobilized on the surface. An effect of the immobilisation method is investigated. Immobilization methods included: (i) chemisorption of aptamers modified with SH groups, (ii) immobilisation of biotin-tagged aptamers via previously immobilized avidin, neutravidin or streptavidin, (iii) aptamer-dendrimer method. Immobilisation of aptamers by the streptavidin-biotin yields the best sensor specificity and sensitivity. (en) Senzor s povrchovými plazmony byl použit ke studiu vlivu imobilizační metody DNA aptameru na povrch senzoru pro detekci trombinu. Byly vyšetřovány následující imobilizační postupy: (a) chemisorpce aptameru pomocí SH skupin, (b) vazba biotinovaného aptameru přes avidin, neutravidin a streptavidin, (c) vazba aptameru na dendrimerovou vrstvu. Nejvyšší citlivosti senzoru a specificity bylo dosaženo za použití imobilizačního postupu streptavidin-biotin. (cs)
Title	Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance (en) Vliv imiobilizace DNA aptamerů na detekci trombinu pomocí senzoru založeném na rezonanční excitaci povrchových plazmonů (cs)
skos:prefLabel	Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance (en) Vliv imiobilizace DNA aptamerů na detekci trombinu pomocí senzoru založeném na rezonanční excitaci povrchových plazmonů (cs)
skos:notation	RIV/67985882:_____/08:00325820!RIV09-AV0-67985882
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(KAN200670701), Z(AV0Z20670512)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Homola, Jiří Vaisocherová, Hana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav fotoniky a elektroniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	365291
http://linked.open...ai/riv/idVysledku	RIV/67985882:_____/08:00325820
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA aptamer; thrombin; dendrimers (en)
http://linked.open.../riv/klicoveSlovo	thrombin dendrimers DNA aptamer
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[7B9373757B5A]
http://linked.open...i/riv/nazevZdroje	Analytical and Bioanalytical Chemistry
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Surface plasmon resonance biosensors and protein arrays for medical diagnostics
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	391
http://linked.open...iv/tvurceVysledku	Homola, Jiří Vaisocherová, Hana Hianik, T. Ostatná, V.
http://linked.open...n/vavai/riv/zamer	Materiály, struktury, systémy a signály v elektronice, optoelektronice a fotonice
issn	1618-2642
number of pages	9 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance Effect of the immobilisation of DNA aptamers on the detection of thrombin by means of surface plasmon resonance

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