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  • Práce se zabývá způsobem stanovení změn pH roztoků s jemným prostorovým rozlišením prostřednictvím zúžených optických vláken a fluorescence. Tento přístup může být využit pro stanovení toku auxinů skrz buněčnou membránu. Byly otestovány absorpční a fluorescenční vlastnosti vybraných pH barviv. Pro zpracování hodnot fluorescence naměřených v roztocích bylo využito posunu maxima fluorescence ve spektru. In vitro byla předvedena použitelnost připravených zúžených optických vláken pro měření pH v rozsahu od 6 do 7. (cs)
  • The paper shows an approach to the determination of pH changes of solutions with a fine spatial resolution by means of fiber-optic tapers and fluorescence detection. This approach can be adopted for the determination of auxin flow through celluar membranes. Spectral absorption and fluorescence of selected pH transducers were tested. The approach, based on the determination of a shift of the maxima of their fluorescence peaks, was employed for processing the measured fluorescence data in bulk solutions.
  • The paper shows an approach to the determination of pH changes of solutions with a fine spatial resolution by means of fiber-optic tapers and fluorescence detection. This approach can be adopted for the determination of auxin flow through celluar membranes. Spectral absorption and fluorescence of selected pH transducers were tested. The approach, based on the determination of a shift of the maxima of their fluorescence peaks, was employed for processing the measured fluorescence data in bulk solutions. (en)
Title
  • Optical principle of pH measurement for detection of auxin flow through cellular membrane
  • Optical principle of pH measurement for detection of auxin flow through cellular membrane (en)
  • Optický princip měření pH pro detekci toku auxinů skrz buněčnou membránu (cs)
skos:prefLabel
  • Optical principle of pH measurement for detection of auxin flow through cellular membrane
  • Optical principle of pH measurement for detection of auxin flow through cellular membrane (en)
  • Optický princip měření pH pro detekci toku auxinů skrz buněčnou membránu (cs)
skos:notation
  • RIV/67985882:_____/07:00086390!RIV08-AV0-67985882
http://linked.open.../vavai/riv/strany
  • 65850Y.1;6580Y.7
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(LC06034), Z(AV0Z20670512)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
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http://linked.open...dnocenehoVysledku
  • 439696
http://linked.open...ai/riv/idVysledku
  • RIV/67985882:_____/07:00086390
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • optical fibres; fluorescence; pH measurement (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [3595592364F3]
http://linked.open...v/mistoKonaniAkce
  • Prague
http://linked.open...i/riv/mistoVydani
  • Bellingham
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  • Optical Sensing Technology and Applications
http://linked.open...in/vavai/riv/obor
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http://linked.open...vavai/riv/projekt
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http://linked.open...iv/tvurceVysledku
  • Aubrecht, Jan
  • Kašík, Ivan
  • Martan, Tomáš
  • Matějec, Vlastimil
  • Mrázek, Jan
  • Podrazký, Ondřej
  • Seidl, Miroslav
  • Tobiška, Petr
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
http://linked.open...n/vavai/riv/zamer
number of pages
http://purl.org/ne...btex#hasPublisher
  • SPIE - The International Society for Optical Engineering
https://schema.org/isbn
  • 978-0-8194-6713-3
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