About: HPLC in Reversed Phase Mode: Tool for Investigation of Kinetics of Blackcurrant Seed Oil Lipolysis in Supercritical Carbon Dioxide     Goto   Sponge   NotDistinct   Permalink

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  • Blackcurrant (Ribes nigrum) seed oil is rich in α- and γ-linolenic acids, the latter in particular beeing of potential use in medicine. The enzymatic hydrolysis of the oil was carried out in supercritical carbon dioxide using lipase Lipozyme as catalyst and changes in the composition of acylglycerols were recorded. Mono-, di-, and triacylglycerols and free fatty acids were separated by non-aqueous high-performance liquid chromatography in reversed phase mode and detected by UV diode array and 1H NMR detectors. Lipozyme was found to exert low specificity to individual fatty acids in the hydrolysed oil.
  • Blackcurrant (Ribes nigrum) seed oil is rich in α- and γ-linolenic acids, the latter in particular beeing of potential use in medicine. The enzymatic hydrolysis of the oil was carried out in supercritical carbon dioxide using lipase Lipozyme as catalyst and changes in the composition of acylglycerols were recorded. Mono-, di-, and triacylglycerols and free fatty acids were separated by non-aqueous high-performance liquid chromatography in reversed phase mode and detected by UV diode array and 1H NMR detectors. Lipozyme was found to exert low specificity to individual fatty acids in the hydrolysed oil. (en)
Title
  • HPLC in Reversed Phase Mode: Tool for Investigation of Kinetics of Blackcurrant Seed Oil Lipolysis in Supercritical Carbon Dioxide
  • HPLC in Reversed Phase Mode: Tool for Investigation of Kinetics of Blackcurrant Seed Oil Lipolysis in Supercritical Carbon Dioxide (en)
skos:prefLabel
  • HPLC in Reversed Phase Mode: Tool for Investigation of Kinetics of Blackcurrant Seed Oil Lipolysis in Supercritical Carbon Dioxide
  • HPLC in Reversed Phase Mode: Tool for Investigation of Kinetics of Blackcurrant Seed Oil Lipolysis in Supercritical Carbon Dioxide (en)
skos:notation
  • RIV/67985858:_____/06:00338608!RIV10-AV0-67985858
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA203/04/0120), Z(AV0Z40720504)
http://linked.open...iv/cisloPeriodika
  • 1-2
http://linked.open...vai/riv/dodaniDat
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  • 478311
http://linked.open...ai/riv/idVysledku
  • RIV/67985858:_____/06:00338608
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  • enzyme; supercritical carbon dioxide; oil hydrolysis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [E92441BD80B5]
http://linked.open...i/riv/nazevZdroje
  • Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
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http://linked.open...v/svazekPeriodika
  • 839
http://linked.open...iv/tvurceVysledku
  • Sýkora, Jan
  • Sovová, Helena
  • Bernášek, Prokop
  • Bártlová, Milena
http://linked.open...ain/vavai/riv/wos
  • 000239299100013
http://linked.open...n/vavai/riv/zamer
issn
  • 1570-0232
number of pages
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