About: Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol-Gels

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this work, we have compared the entrapment of free or immobilized on a porous support (Sepabeads using glutaraldehyde technique) glucose oxidase using a sol-gel technique. The previous immobilization of the enzyme permitted to keep the enzyme activity after sol-gel formation (only 10% of the enzyme activity was lost whereas the soluble enzyme lost 60% of its initial activity) and to avoid enzyme leakage from the sol-gel matrix, which was relatively high when entrapping the soluble enzyme (39 % of the enzyme activity was released after 16 hr of incubation in a buffered solution). Moreover, the immobilized enzyme, inside the porous support, cannot be in contact with the sol-gel and maintained the stability achieved by multipoint covalent attachment on the Sepabeads, giving much better stability than the one achieved by trapping the soluble enzyme. In this work, we have compared the entrapment of free or immobilized on a porous support (Sepabeads using glutaraldehyde technique) glucose oxidase using a sol-gel technique. The previous immobilization of the enzyme permitted to keep the enzyme activity after sol-gel formation (only 10% of the enzyme activity was lost whereas the soluble enzyme lost 60% of its initial activity) and to avoid enzyme leakage from the sol-gel matrix, which was relatively high when entrapping the soluble enzyme (39 % of the enzyme activity was released after 16 hr of incubation in a buffered solution). Moreover, the immobilized enzyme, inside the porous support, cannot be in contact with the sol-gel and maintained the stability achieved by multipoint covalent attachment on the Sepabeads, giving much better stability than the one achieved by trapping the soluble enzyme. (en) V této práci bylo porovnáváno zachycení glucosa oxidasy volné a předimobilizované na Sepabeads do křemičitého gelu. V případě imobilizace volného enzymu aktivita byla sníženo o 60% a docházelo ke ztrátám enzymu únikem z nosiče (39% během 16 hodin). Aktivity přeimobilizovaného enzymu byla snížena pouye o 10%. Mnohabodové kovalentní vázání enyzmu na Sepabeads zvyšuje stabilitu enzymu a zybraňuje jeho úniku. (cs)
Title	Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol-Gels Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol-Gels (en) Výhody předimobilizace enyzmů na porézním nosiči pro jejich zachycení v sol-gelu (cs)
skos:prefLabel	Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol-Gels Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol-Gels (en) Výhody předimobilizace enyzmů na porézním nosiči pro jejich zachycení v sol-gelu (cs)
skos:notation	RIV/67985858:_____/05:00028621!RIV06-AV0-67985858
http://linked.open.../vavai/riv/strany	1027;1030
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z40720504)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Podrazký, Ondřej Kuncová, Gabriela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav chemických procesů AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	511469
http://linked.open...ai/riv/idVysledku	RIV/67985858:_____/05:00028621
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	immobilization; enzymes; sol-gel (en)
http://linked.open.../riv/klicoveSlovo	enzymes sol-gel immobilization
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[76B92BA04B39]
http://linked.open...i/riv/nazevZdroje	Biomacromolecules
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Kuncová, Gabriela Podrazký, Ondřej Fuentes, M. Betancor, L. Fernández-Lafuente, R. Guisán, J. M. Hidalgo, A. López-Gallego, F.
http://linked.open...n/vavai/riv/zamer	Investigation of multiphase reacting systems for the design of processes important in synthesis and preparation of novel materials, in energy production and in environmental protection
issn	1525-7797
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Advantages of the Pre-Immobilization of Enzymes on Porous Supports for Their Entrapment in Sol-Gels

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