| Attributes | Values |
|---|
| rdf:type
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| Description
| - Proteomic analysis was performed in post-nuclear supernatant (PNS) and Percoll-purified membranes (PM) prepared from frontal brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days. In the PNS, the 10 up- or down-regulated proteins exhibiting the largest morphine-induced change were selected, excised manually from the gel and identified by MALDI-TOF MS/MS. The identified proteins were of cytoplasmic, cell membrane, endoplasmic reticulum and mitochondrial origin and 9 of them were significantly increased. In PM, the 18 up- or down-regulated proteins were identified by LC-MS/MS and were of plasma membrane, myelin membrane, cytoplasmic and mitochondrial origin. The only member of GPCR-initiated signaling cascades identified in PM as altered by morphine was Gβ subunit. Surprisingly, the immunoblot analysis of the same PM indicated that the “active, morphine-induced pool of Gβ subunits, represented just a minor fraction of the total signal of Gβ which was decreased 1.2x only. An alteration of proteins functionally related to genesis of oxidative stress, apoptosis and energy metabolism suggests that the brain cortex of rats exposed to increasing doses of morphine can not be regarded as fully adapted to morphine
- Proteomic analysis was performed in post-nuclear supernatant (PNS) and Percoll-purified membranes (PM) prepared from frontal brain cortex of rats exposed to increasing doses of morphine (10-50 mg/kg) for 10 days. In the PNS, the 10 up- or down-regulated proteins exhibiting the largest morphine-induced change were selected, excised manually from the gel and identified by MALDI-TOF MS/MS. The identified proteins were of cytoplasmic, cell membrane, endoplasmic reticulum and mitochondrial origin and 9 of them were significantly increased. In PM, the 18 up- or down-regulated proteins were identified by LC-MS/MS and were of plasma membrane, myelin membrane, cytoplasmic and mitochondrial origin. The only member of GPCR-initiated signaling cascades identified in PM as altered by morphine was Gβ subunit. Surprisingly, the immunoblot analysis of the same PM indicated that the “active, morphine-induced pool of Gβ subunits, represented just a minor fraction of the total signal of Gβ which was decreased 1.2x only. An alteration of proteins functionally related to genesis of oxidative stress, apoptosis and energy metabolism suggests that the brain cortex of rats exposed to increasing doses of morphine can not be regarded as fully adapted to morphine (en)
|
| Title
| - Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine
- Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine (en)
|
| skos:prefLabel
| - Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine
- Proteomic analysis of post-nuclear supernatant fraction and percoll-purified membranes prepared from brain cortex of rats exposed to increasing doses of morphine (en)
|
| skos:notation
| - RIV/67985823:_____/14:00427791!RIV15-GA0-67985823
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - I, P(GAP207/12/0919), P(GBP304/12/G069)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/67985823:_____/14:00427791
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - morphine; long-term exposure; rat brain cortex; isolated plasma membranes; post-nuclear supernatant; 2D electrophoresis (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
|
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Svoboda, Petr
- Kagan, Dmytro
- Eckhardt, Adam
- Roubalová, Lenka
- Ujčíková, Hana
|
| http://linked.open...ain/vavai/riv/wos
| |
| issn
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| number of pages
| |
| http://bibframe.org/vocab/doi
| |
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