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Description
| - We describe a protocol for high-efficiency germline transgenesis and sustained transgene expression in pigs by using the Sleeping Beauty (SB) transposon system. The protocol is based on co-injection of a plasmid encoding the SB100X hyperactive transposase, together with a second plasmid carrying a transgene flanked by binding sites for the transposase, into the cytoplasm of porcine zygotes
- We describe a protocol for high-efficiency germline transgenesis and sustained transgene expression in pigs by using the Sleeping Beauty (SB) transposon system. The protocol is based on co-injection of a plasmid encoding the SB100X hyperactive transposase, together with a second plasmid carrying a transgene flanked by binding sites for the transposase, into the cytoplasm of porcine zygotes (en)
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Title
| - Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons
- Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons (en)
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skos:prefLabel
| - Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons
- Germline transgenesis in pigs by cytoplasmic microinjection of Sleeping Beauty transposons (en)
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skos:notation
| - RIV/67985823:_____/14:00427711!RIV15-TA0-67985823
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/67985823:_____/14:00427711
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - pig; transgenic; Sleeping Beauty; protocol (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Landa, Vladimír
- Pravenec, Michal
- Zídek, Václav
- Rülicke, T.
- Bashir, S.
- Ivics, Z.
- Izsvák, Z.
- Mátés, L.
- Garrels, W.
- Geurts, A.
- Kues, W. A.
- Yau, T. Y.
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
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