About: FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion

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About: FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The observation that 22-NBD-cholesterol appears to be indifferent to the changes in the membrane packing in living cells is in agreement with previous studies in model membranes. However, our data indicate that the alternation of plasma membrane structure induced by decrease of cholesterol level by beta-CDX makes the membrane environment of NBD moiety of 25-NBD-cholesterol probe a significantly more hydrated. This finding not only encourages using 25-NBD-cholesterol in living cells, but also demonstrates that previously drawn discouraging conclusions on the use of 25-NBD-cholesterol in model membranes are not valid for living cells The observation that 22-NBD-cholesterol appears to be indifferent to the changes in the membrane packing in living cells is in agreement with previous studies in model membranes. However, our data indicate that the alternation of plasma membrane structure induced by decrease of cholesterol level by beta-CDX makes the membrane environment of NBD moiety of 25-NBD-cholesterol probe a significantly more hydrated. This finding not only encourages using 25-NBD-cholesterol in living cells, but also demonstrates that previously drawn discouraging conclusions on the use of 25-NBD-cholesterol in model membranes are not valid for living cells (en)
Title	FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion (en)
skos:prefLabel	FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletion (en)
skos:notation	RIV/67985823:_____/13:00391710!RIV14-GA0-67985823
http://linked.open...avai/predkladatel	Fyziologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GAP207/12/0919), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika	FEB-MAR
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Svoboda, Petr Ostašov, Pavel Brejchová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	75296
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/13:00391710
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cholesterol depletion; beta-Cyclodextrin; 22-NBD-cholesterol; 25-NBD-cholesterol; FLIM studies; intact HEK293 cells (en)
http://linked.open.../riv/klicoveSlovo	cholesterol depletion beta-Cyclodextrin 22-NBD-cholesterol 25-NBD-cholesterol FLIM studies intact HEK293 cells
http://linked.open...odStatuVydavatele	IE - Irsko
http://linked.open...ontrolniKodProRIV	[A5B73BF02029]
http://linked.open...i/riv/nazevZdroje	Chemistry and Physics of Lipids
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	The role of hydrophobic plasma membrane interior in DOR regulation of trimeric G protein activity
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	167-168
http://linked.open...iv/tvurceVysledku	Hof, Martin Svoboda, Petr Sýkora, Jan Olžyńska, Agnieszka Brejchová, Jana Ostašov, Pavel
http://linked.open...ain/vavai/riv/wos	000318137600008
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka
issn	0009-3084
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.chemphyslip.2013.02.006

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