About: The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein

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About: The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effect of monovalent cations on trimeric G protein Gi1alfa was measured at equimolar concentration of chloride anion in pertussis-toxin (PTX)-treated HEK293 cells stably expressing PTX-insensitive DOR-Gi1alfa (Cys351-Ile351) fusion protein by high-affinity [35S]GTPgamaS binding assay. The high basal level of binding was detected in absence of DOR agonist and monovalent ions and this high level was inhibited with the order of: Naplus je větší než Kplus je větší nez Liplus. The inhibition by monovalent ions was reversed by increasing concentrations of DOR agonist DADLE. The maximum DADLE response was also highest for sodium and decreased in the order of: Naplus je větší než Kplus rovná se Liplus. Our data indicate i) an inherently high activity of trimeric G protein Gi1alfa when expressed within DOR-Gi1alfa fusion protein and determined in the absence of monovalent cations, ii) preferential sensitivity of DOR-Gi1alfa to sodium as far as maximum of agonist response is involved The effect of monovalent cations on trimeric G protein Gi1alfa was measured at equimolar concentration of chloride anion in pertussis-toxin (PTX)-treated HEK293 cells stably expressing PTX-insensitive DOR-Gi1alfa (Cys351-Ile351) fusion protein by high-affinity [35S]GTPgamaS binding assay. The high basal level of binding was detected in absence of DOR agonist and monovalent ions and this high level was inhibited with the order of: Naplus je větší než Kplus je větší nez Liplus. The inhibition by monovalent ions was reversed by increasing concentrations of DOR agonist DADLE. The maximum DADLE response was also highest for sodium and decreased in the order of: Naplus je větší než Kplus rovná se Liplus. Our data indicate i) an inherently high activity of trimeric G protein Gi1alfa when expressed within DOR-Gi1alfa fusion protein and determined in the absence of monovalent cations, ii) preferential sensitivity of DOR-Gi1alfa to sodium as far as maximum of agonist response is involved (en)
Title	The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein (en)
skos:prefLabel	The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein (en)
skos:notation	RIV/67985823:_____/11:00370263!RIV12-AV0-67985823
http://linked.open...avai/predkladatel	Fyziologický ústav AV ČR, v.v.i.
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD305/08/H037), P(IAA500110606), P(LC554), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Svoboda, Petr Vošahlíková, Miroslava
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	204486
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/11:00370263
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	delta-opioid receptor (DOR); monovalent ions; G(i)1alfa protein (en)
http://linked.open.../riv/klicoveSlovo	delta-opioid receptor (DOR) G(i)1alfa protein monovalent ions
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[3729398F90B3]
http://linked.open...i/riv/nazevZdroje	Physiological Research
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Physiology of animal cell (2) Neuroscience Centre Delta-opioid receptor targeting to membrane domains: the role of palmitoylation
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Svoboda, Petr Vošahlíková, Miroslava
http://linked.open...ain/vavai/riv/wos	000293138600015
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka
issn	0862-8408
number of pages	7 (xsd:int)
is http://linked.open...avai/riv/vysledek of	The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein

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