About: Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy

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About: Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	S využitím 4Pi mikroskopie jsme sledovali 3D morfologii mitochondriální sítě a její změny v závislosti na stupni oxidativní fosforylace u INS-1E a HEP-G2 buněk v bezprecedentní detailní úrovni 100 nm. Jediná mitochondrie, retikulární síť byla nalezena u INS-1E buněk s průměrem tubulů 262+26 nm. Rotenon inhibující respiraci na zbylých 10% dezintegroval tuto síť na několik částí a početné kuličky a krátké segmenty. Odpřažení vedlo navíc k tvorbě prstenců. Rotenon při odpřažení ještě zvýšil fúzování objektů do tvorby miskovitých útvarů. Odhalili jsme tak dříve nepopsané tvary mitochondriální sítě a zjistli, že 3D mikroskopie s vysokým rozlišením je nutné pro její studium (cs) Using 4Pi microscopy, 3D morphology changes of mitochondrial reticulum network responding to decreased oxidative phosphorylation have been observed in INS-1E and HEP-G2 cells at an unprecedented 100 nm level of detail. In INS-1E cells a single interconnected mitochondrion with a nearly constant 262+26 nm tubule diameter was found. With rotenone inhibiting respiration to 10%, disintegration into several reticula and numerous 300 nm spheres or short tubules was observed. De-energization by uncoupling additionally led to formation of rings and bulky cisternae of 1.4+0.4 μm diameter. Rotenone and uncoupler acted synergically in INS-1E cells and increased fusion (ongoing with fission) forming bowl-like shapes. In HEP-G2 cells fission partially ceased with FCCP plus rotenone. Thus we have revealed previously undescribed details for shapes upon mitochondrial disintegration and clearly demonstrate that high resolution 3D microscopy is required for visualization of mitochondrial network Using 4Pi microscopy, 3D morphology changes of mitochondrial reticulum network responding to decreased oxidative phosphorylation have been observed in INS-1E and HEP-G2 cells at an unprecedented 100 nm level of detail. In INS-1E cells a single interconnected mitochondrion with a nearly constant 262+26 nm tubule diameter was found. With rotenone inhibiting respiration to 10%, disintegration into several reticula and numerous 300 nm spheres or short tubules was observed. De-energization by uncoupling additionally led to formation of rings and bulky cisternae of 1.4+0.4 μm diameter. Rotenone and uncoupler acted synergically in INS-1E cells and increased fusion (ongoing with fission) forming bowl-like shapes. In HEP-G2 cells fission partially ceased with FCCP plus rotenone. Thus we have revealed previously undescribed details for shapes upon mitochondrial disintegration and clearly demonstrate that high resolution 3D microscopy is required for visualization of mitochondrial network (en)
Title	Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy (en) Oxidativní fosforylace a energetický status jsou reflektovány morfologií mitochondriální sítě u INS-1E a HEP-G2 buněk zobrazených 4Pi mikroskopií (cs)
skos:prefLabel	Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy (en) Oxidativní fosforylace a energetický status jsou reflektovány morfologií mitochondriální sítě u INS-1E a HEP-G2 buněk zobrazených 4Pi mikroskopií (cs)
skos:notation	RIV/67985823:_____/08:00314590!RIV09-MZ0-67985823
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GP303/05/P100), P(IAA500110701), P(NR7917), P(NR9183), Z(AV0Z50110509)
http://linked.open...iv/cisloPeriodika	7-8
http://linked.open...vai/riv/dodaniDat	2009
http://linked.open...aciTvurceVysledku	Ježek, Petr Šantorová, Jitka Plecitá-Hlavatá, Lydie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Fyziologický ústav AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	379649
http://linked.open...ai/riv/idVysledku	RIV/67985823:_____/08:00314590
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	4Pi microscopy; 100 nm 3D imaging; mitochondrial network (en)
http://linked.open.../riv/klicoveSlovo	4Pi microscopy 100 nm 3D imaging mitochondrial network
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[BE43B8692F58]
http://linked.open...i/riv/nazevZdroje	Biochimica Et Biophysica Acta-Bioenergetics
http://linked.open...in/vavai/riv/obor	EA
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Antioxidant role vs. mitochondrial morphology and their impairment during oxidative stress in diabetic tissues and ischemic heart Mitochondrial morphology a its changes in diabetes Significance of mitochondrial uncoupling in pancreatic beta cells for development of type-2 diabetes. Mitochondrial control of reactive oxygen species in cytosol and extracellular space
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	1777
http://linked.open...iv/tvurceVysledku	Ježek, Petr Bewersdorf, J. Šantorová, Jitka Hlavatá, Lydie Lessard, M.
http://linked.open...ain/vavai/riv/wos	257696400039
http://linked.open...n/vavai/riv/zamer	Výzkum molekulárních a buněčných základů fyziologických a patofyziologických procesů s cílem objasnit mechanismy vzniku závažných onemocnění člověka
issn	0005-2728
number of pages	13 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy Mitochondrial oxidative phosphorylation and energetic status are reflected by morphology of mitochondrial network in INS-1E and HEP-G2 cells viewed by 4Pi microscopy

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