About: Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation

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About: Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	BACKGROUND: Food and feed technology and biotechnology benefit from the use of immobilised enzymes. New low-cost enzyme carriers exhibiting high biocompatibility and response to external magnetic field can substantially improve the application potential of immobilised enzyme systems. RESULTS: Ferrofluid-modified spent grain was used as a low-cost, biocompatible and magnetically responsive carrier for the immobilisation of Candida rugosa lipase. Several immobilisation procedures were tested using both native and poly(ethyleneimine)-modified magnetic spent grain. Activity of immobilised lipase per unit mass of carrier, operational stability, time stability and Michaelis constant were compared. In general, magnetic spent grain modified with poly(ethyleneimine) bound a smaller amount of active lipase than unmodified magnetic spent grain, but the operational and storage stabilities of enzyme immobilised on poly(ethyleneimine)-modified carrier were very high. CONCLUSION: Ferrofluid-modified spent grain can be a promising low-cost magnetic carrier for enzyme immobilisation, applicable e.g. in food and feed technology and biotechnology. BACKGROUND: Food and feed technology and biotechnology benefit from the use of immobilised enzymes. New low-cost enzyme carriers exhibiting high biocompatibility and response to external magnetic field can substantially improve the application potential of immobilised enzyme systems. RESULTS: Ferrofluid-modified spent grain was used as a low-cost, biocompatible and magnetically responsive carrier for the immobilisation of Candida rugosa lipase. Several immobilisation procedures were tested using both native and poly(ethyleneimine)-modified magnetic spent grain. Activity of immobilised lipase per unit mass of carrier, operational stability, time stability and Michaelis constant were compared. In general, magnetic spent grain modified with poly(ethyleneimine) bound a smaller amount of active lipase than unmodified magnetic spent grain, but the operational and storage stabilities of enzyme immobilised on poly(ethyleneimine)-modified carrier were very high. CONCLUSION: Ferrofluid-modified spent grain can be a promising low-cost magnetic carrier for enzyme immobilisation, applicable e.g. in food and feed technology and biotechnology. (en)
Title	Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation (en)
skos:prefLabel	Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation Magnetically modified spent grain as a low-cost, biocompatible and smart carrier for enzyme immobilisation (en)
skos:notation	RIV/67179843:_____/13:00398937!RIV14-GA0-67179843
http://linked.open...avai/predkladatel	Centrum výzkumu globální změny AV ČR v.v.i.
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(GAP503/11/2263), P(LH12190)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Šafařík, Ivo
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	85733
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/13:00398937
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	magnetic fluid; spent grain; lipase; magnetic carrier; immobilisation (en)
http://linked.open.../riv/klicoveSlovo	magnetic carrier lipase magnetic fluid immobilisation spent grain
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[988D15C03D63]
http://linked.open...i/riv/nazevZdroje	Journal of the Science of Food and Agriculture
http://linked.open...in/vavai/riv/obor	EI
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Magnetic nano- and microparticles for advanced membrane water treatment Magnetic (bio)composite materials for separation of target compounds, heavy metals and radionuclides
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	93
http://linked.open...iv/tvurceVysledku	Šafařík, Ivo Pospišková, K.
http://linked.open...ain/vavai/riv/wos	000317614000010
issn	0022-5142
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1002/jsfa.5930

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