About: Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803

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About: Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803 Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	State transitions in cyanobacteria are physiological adaptation mechanisms that change the interaction of the phycobilisomes with the photosystem I and photosystem II core complexes. Previous studies of cyanobacteria have identified a gene named rpaC, which appears to be specifically required for state transitions. The gene product of rpaC is very probably a transmembrane protein that is a structural component of the phycobilisome-photosystem II supercomplex. Here we report the construction of an expression system that enables high production of fusion protein TrxHisTagSTag-RpaC, and describe suitable conditions for purification of this insoluble protein at a yield of 3 mg per 1 dm3 of bacterial culture. State transitions in cyanobacteria are physiological adaptation mechanisms that change the interaction of the phycobilisomes with the photosystem I and photosystem II core complexes. Previous studies of cyanobacteria have identified a gene named rpaC, which appears to be specifically required for state transitions. The gene product of rpaC is very probably a transmembrane protein that is a structural component of the phycobilisome-photosystem II supercomplex. Here we report the construction of an expression system that enables high production of fusion protein TrxHisTagSTag-RpaC, and describe suitable conditions for purification of this insoluble protein at a yield of 3 mg per 1 dm3 of bacterial culture. (en)
Title	Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803 Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803 (en)
skos:prefLabel	Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803 Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803 (en)
skos:notation	RIV/67179843:_____/09:00341135!RIV13-GA0-67179843
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(GA206/07/0917), S, Z(AV0Z60870520), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	47
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Cséfalvay, Eva Komárek, Ondřej Lapkouski, Mikalai
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	314432
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/09:00341135
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	affinity chromatography; photosystem; phycobilisome – PSII supercomplex (en)
http://linked.open.../riv/klicoveSlovo	affinity chromatography photosystem phycobilisome – PSII supercomplex
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[83295C11C1B5]
http://linked.open...i/riv/nazevZdroje	Photosynthetica
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Regulation and adaptive mechanisms of aerophytic algae and cyanobacteria in ambient semiarid conditions
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	3
http://linked.open...iv/tvurceVysledku	Komárek, Ondřej Lapkouski, Mikalai Cséfalvay, Eva
http://linked.open...ain/vavai/riv/wos	000271672900006
http://linked.open...n/vavai/riv/zamer	Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	0300-3604
number of pages	8 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Expression, purification and preliminary crystallization study of RpaC protein from Synechocystis sp PCC6803

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