About: Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803

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About: Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803 Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Protein PsbH byl exprimován jako fúzní protein s GST kotvou v normálním médiu a v médiu obohaceném 15N. Byla provedena měření CD a NMR v roztocích s detergentem OG. Byla stanovena struktura 15% ? skládaný list, 38% helix, zbytek ? obrat. Bylo proveden výpočet struktury mimomembránových součástí proteinu, který, při započtení vnitromembránového helixu, dal hodnoty strukrturních motivů ve shodě s experimentem. (cs) The PsbH protein of cyanobacterium Synechocystis sp. PCC 6803 was expressed as a fusion protein with glutathione-S transferase (GST) in E. coli grown on a mineral medium enriched in 15 N isotope. After enzymatic cleavage of the fusion protein, the H-1-N-15-HSQC spectrum of PsbH protein in presence of the detergent P-D-octyl-glucopyranoside (OG) was recorded on a Bruker DRX 500 MHz NMR spectrometer equipped with a 5 mm TXI cryoprobe to enhance the sensitivity and resolution. Non-labelled protein was used for secondary structure estimation by de-convolution from circular dichroism (CD) spectra. Experimental results were compared with our results from a structural model of PsbH using a restraint-based comparative modelling approach combined with molecular dynamics and energetic modelling. We found that PsbH shows 34-38% alpha-helical structure (ThrM-Ser60), a maximum of around 15 % of beta-sheet,and 12-19 % of P-turn. The PsbH protein of cyanobacterium Synechocystis sp. PCC 6803 was expressed as a fusion protein with glutathione-S transferase (GST) in E. coli grown on a mineral medium enriched in 15 N isotope. After enzymatic cleavage of the fusion protein, the H-1-N-15-HSQC spectrum of PsbH protein in presence of the detergent P-D-octyl-glucopyranoside (OG) was recorded on a Bruker DRX 500 MHz NMR spectrometer equipped with a 5 mm TXI cryoprobe to enhance the sensitivity and resolution. Non-labelled protein was used for secondary structure estimation by de-convolution from circular dichroism (CD) spectra. Experimental results were compared with our results from a structural model of PsbH using a restraint-based comparative modelling approach combined with molecular dynamics and energetic modelling. We found that PsbH shows 34-38% alpha-helical structure (ThrM-Ser60), a maximum of around 15 % of beta-sheet,and 12-19 % of P-turn. (en)
Title	Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803 Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803 (en) Určení sekundární struktury rekombinantního psbH, fotosyntetického membránového proteinu ze sinice Synechocystis sp. PCC 6803 (cs)
skos:prefLabel	Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803 Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803 (en) Určení sekundární struktury rekombinantního psbH, fotosyntetického membránového proteinu ze sinice Synechocystis sp. PCC 6803 (cs)
skos:notation	RIV/67179843:_____/05:00029260!RIV06-AV0-67179843
http://linked.open.../vavai/riv/strany	421;424
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z60870520), Z(MSM6007665808)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Štys, Dalibor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Centrum výzkumu globální změny AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	542062
http://linked.open...ai/riv/idVysledku	RIV/67179843:_____/05:00029260
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	protein (en)
http://linked.open.../riv/klicoveSlovo	protein
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[E250E727DCFE]
http://linked.open...i/riv/nazevZdroje	Photosynthetica
http://linked.open...in/vavai/riv/obor	CF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	43
http://linked.open...iv/tvurceVysledku	Štys, Dalibor
http://linked.open...n/vavai/riv/zamer	Spatial and functional dynamics of biological, ecological and socio-economic systems interacting with global climatic change Fyzikální biologie -nové přístupy v biologickém výzkumu
issn	0300-3604
number of pages	4 (xsd:int)
is http://linked.open...avai/riv/vysledek of	Secondary structure estimation of recombinant psbH, encoding a photosynthetic membrane protein of cyanobacterium Synechocystis sp. PCC 6803

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