About: Effect of ABCG2 on cytotoxicity of platinum drugs: Interference of EGFP

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About: Effect of ABCG2 on cytotoxicity of platinum drugs: Interference of EGFP Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	ATP-binding drug efflux transporters decrease intracellular concentrations of cytotoxic drugs, causing multidrug resistance in cancer. In this study, we examined possible interactions of ABCG2 transporter with platinum cytotoxic drugs. We demonstrate here an interference of platinum drugs with enhanced green fluorescence protein (EGFP) in the cellular models, where EGFP was employed as a reporter gene. Cytotoxicity of cisplatin (CIP), carboplatin (CAP) and oxaliplatin (OXP) was significantly lowered in MDCKII cells transfected with ABCG2 transporter and EGFP reporter. The IC(50) values in MDCKII-ABCG2 were 25.7, 164 and 165 microM for CIP, CAP and OXP, respectively, whereas IC(50) for the same cytostatics in MDCKII cells were as follows: 15.4, 133 and 50.3 microM. Addition of fumitremorgin C (FTC), a potent ABCG2 inhibitor, significantly suppressed the resistance of MDCKII-ABCG2 to OXP, suggesting that OXP interacts with ABCG2. However, FTC did not change the sensitivity of the cells to CIP and CAP. ATP-binding drug efflux transporters decrease intracellular concentrations of cytotoxic drugs, causing multidrug resistance in cancer. In this study, we examined possible interactions of ABCG2 transporter with platinum cytotoxic drugs. We demonstrate here an interference of platinum drugs with enhanced green fluorescence protein (EGFP) in the cellular models, where EGFP was employed as a reporter gene. Cytotoxicity of cisplatin (CIP), carboplatin (CAP) and oxaliplatin (OXP) was significantly lowered in MDCKII cells transfected with ABCG2 transporter and EGFP reporter. The IC(50) values in MDCKII-ABCG2 were 25.7, 164 and 165 microM for CIP, CAP and OXP, respectively, whereas IC(50) for the same cytostatics in MDCKII cells were as follows: 15.4, 133 and 50.3 microM. Addition of fumitremorgin C (FTC), a potent ABCG2 inhibitor, significantly suppressed the resistance of MDCKII-ABCG2 to OXP, suggesting that OXP interacts with ABCG2. However, FTC did not change the sensitivity of the cells to CIP and CAP. (en)
Title	Effect of ABCG2 on cytotoxicity of platinum drugs: Interference of EGFP Effect of ABCG2 on cytotoxicity of platinum drugs: Interference of EGFP (en)
skos:prefLabel	Effect of ABCG2 on cytotoxicity of platinum drugs: Interference of EGFP Effect of ABCG2 on cytotoxicity of platinum drugs: Interference of EGFP (en)
skos:notation	RIV/63221667:_____/08:#0000022!RIV10-MZ0-63221667
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(1A8696), S
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Vacková, Zuzana Radilová, Hana Libra, Antonín Bunček, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	GENERI BIOTECH s.r.o.
http://linked.open...dnocenehoVysledku	364985
http://linked.open...ai/riv/idVysledku	RIV/63221667:_____/08:#0000022
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Platinum cytostatics; ABCG2; MTT assay; Neutral red assay; Cytotoxicity; Apoptosis; EGFP (en)
http://linked.open.../riv/klicoveSlovo	MTT assay Apoptosis Cytotoxicity EGFP Neutral red assay Platinum cytostatics ABCG2
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[C31E48F860D2]
http://linked.open...i/riv/nazevZdroje	Toxicology in Vitro
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	In-vitro study of molecular-genetic cuase of cell resistance against selected drugs
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	22
http://linked.open...iv/tvurceVysledku	Čečková, Martina Štaud, František Bunček, Martin Libra, Antonín Vacková, Zuzana Radilová, Hana
issn	0887-2333
number of pages	7 (xsd:int)

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