About: A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic

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About: A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic Goto Sponge NotDistinct Permalink

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Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://www.sciencedirect.com/science/article/pii/S0278691513006480
Description	Ochratoxin A (OTA) is one of the most naturally occurring fungal toxins in food. It has been detected in high concentrations in serum samples of nephropathic patients and can be applied as one of the markers of potential risk of this disease. Also, OTA can cause adverse effects on human health such as genotoxicity and is anticipated to be a potential human carcinogen. In this study, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were applied in analysis of 115 blood serum samples of women in the child rearing age from the Czech Republic and both methods were compared. The OTA was presented in a broad range of concentrations from 0,037 to 1,130 ug/L. The outcome of ELISA and HPLC measurements were well correlated (r = 0.907). However, it was observed that ELISA, tend to result in underestimating the OTA level at the low serum concentrations. Both methods had the same limits of quantification of 0.050ug/L under standard operation conditions. When OTA concentration in a sample was too low, the sample was redissolved in only 300 uL of methanol and the detection limit for HPLC was lowered to 0.030 ug OTA/L. Ochratoxin A (OTA) is one of the most naturally occurring fungal toxins in food. It has been detected in high concentrations in serum samples of nephropathic patients and can be applied as one of the markers of potential risk of this disease. Also, OTA can cause adverse effects on human health such as genotoxicity and is anticipated to be a potential human carcinogen. In this study, enzyme-linked immunosorbent assay (ELISA) and high performance liquid chromatography (HPLC) were applied in analysis of 115 blood serum samples of women in the child rearing age from the Czech Republic and both methods were compared. The OTA was presented in a broad range of concentrations from 0,037 to 1,130 ug/L. The outcome of ELISA and HPLC measurements were well correlated (r = 0.907). However, it was observed that ELISA, tend to result in underestimating the OTA level at the low serum concentrations. Both methods had the same limits of quantification of 0.050ug/L under standard operation conditions. When OTA concentration in a sample was too low, the sample was redissolved in only 300 uL of methanol and the detection limit for HPLC was lowered to 0.030 ug OTA/L. (en)
Title	A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic (en)
skos:prefLabel	A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic A comparison of ELISA and HPLC methods for determination of ochratoxin A in human blood serum in the Czech Republic (en)
skos:notation	RIV/62690094:18470/13:50001708!RIV14-MSM-18470___
http://linked.open...avai/predkladatel	Přírodovědecká fakulta
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...iv/cisloPeriodika	12.09.2013
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Dohnal, Vlastimil Malíř, František Dvořák, Vladimír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Hradec Králové / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	58501
http://linked.open...ai/riv/idVysledku	RIV/62690094:18470/13:50001708
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Serum; HPLC; ELISA; Ochratoxin A (en)
http://linked.open.../riv/klicoveSlovo	ELISA HPLC Ochratoxin A Serum
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[3EF9159920D0]
http://linked.open...i/riv/nazevZdroje	Food and chemical toxicology
http://linked.open...in/vavai/riv/obor	DN
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	62
http://linked.open...iv/tvurceVysledku	Dohnal, Vlastimil Ostrý, Vladimír Dvořák, Vladimír Malíř, František Roubal, Tomáš
http://linked.open...ain/vavai/riv/wos	000329960400056
issn	0278-6915
number of pages	5 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.fct.2013.09.010
http://localhost/t...ganizacniJednotka	18470

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