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Description
| - Hydroxycinnamic acids (HCAs) possess numerous biological and due to these properties are widely used in folk medicine. Nevertheless, they can interact with protein molecules and cause some structural and functional changes. The possibility of HCAs binding to bovine serum albumin (BSA) under physiological conditions was investigated by the UV-VIS absorption spectroscopy, fluorescence quenching method, and FRET method. The binding constants, number of binding sites and free energy changes were determined. The binding affinities of HCAs were ranked in the order: chlorogenic acid > sinapic acid >= caffeic acid > ferulic acid > o-coumaric acid > p-coumaric acid >= m-coumaric acid, which was confirmed by spectral overlaps of BSA emission spectrum with absorption spectrum of HCA. All free energy changes possessed negative sign indicating the spontaneity of HCA-BSA interaction.
- Hydroxycinnamic acids (HCAs) possess numerous biological and due to these properties are widely used in folk medicine. Nevertheless, they can interact with protein molecules and cause some structural and functional changes. The possibility of HCAs binding to bovine serum albumin (BSA) under physiological conditions was investigated by the UV-VIS absorption spectroscopy, fluorescence quenching method, and FRET method. The binding constants, number of binding sites and free energy changes were determined. The binding affinities of HCAs were ranked in the order: chlorogenic acid > sinapic acid >= caffeic acid > ferulic acid > o-coumaric acid > p-coumaric acid >= m-coumaric acid, which was confirmed by spectral overlaps of BSA emission spectrum with absorption spectrum of HCA. All free energy changes possessed negative sign indicating the spontaneity of HCA-BSA interaction. (en)
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Title
| - Binding of naturally occurring hydroxycinnamic acids to bovine serum albumin
- Binding of naturally occurring hydroxycinnamic acids to bovine serum albumin (en)
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skos:prefLabel
| - Binding of naturally occurring hydroxycinnamic acids to bovine serum albumin
- Binding of naturally occurring hydroxycinnamic acids to bovine serum albumin (en)
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skos:notation
| - RIV/62690094:18470/10:00003037!RIV11-MSM-18470___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62690094:18470/10:00003037
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - bovine serum albumin; hydroxycinnamic acid; fluorescence quenching; protein-ligand binding (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Boušová, Iva
- Dršata, Jaroslav
- Trnková, Lucie
- Kubíček, Vladimír
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http://localhost/t...ganizacniJednotka
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