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  • A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, D-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo-and D-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12 min at 10 degrees C, though higher temperatures (25 degrees C or 40 degrees C) or higher water content in the mobile phase could speed up the separation and determination to four minutes. LOD of the method was 9 mg/L at 10 degrees C, and 5 mg/L at 25 degrees C, resp.
  • A fast liquid chromatography method for separation and determination of myo-inositol is reported. Determination of the biologically important isomer of inositols, myo-inositol, was optimized to avoid overlapping to possible interferents according to European Pharmacopoeia (glycerol, D-mannitol) and saccharose. The method in HILIC mode is extremely selective to other carbohydrates which allows to separate myo-inositol from allo-and D-chiro-inositol with resolution 12.3 and 5.2, resp. and this way it enables to separate myo-inostiol from contingent carbohydrates present in a sample matrix. Retention time of myo-inositol was 12 min at 10 degrees C, though higher temperatures (25 degrees C or 40 degrees C) or higher water content in the mobile phase could speed up the separation and determination to four minutes. LOD of the method was 9 mg/L at 10 degrees C, and 5 mg/L at 25 degrees C, resp. (en)
Title
  • Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography
  • Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography (en)
skos:prefLabel
  • Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography
  • Fast separation and determination of free myo-inositol by hydrophilic liquid chromatography (en)
skos:notation
  • RIV/62157124:16370/14:43872716!RIV15-MSM-16370___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S
http://linked.open...iv/cisloPeriodika
  • JUN 2014
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 16515
http://linked.open...ai/riv/idVysledku
  • RIV/62157124:16370/14:43872716
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • ELSD; HILIC; myo-inositol (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [6268E296BB99]
http://linked.open...i/riv/nazevZdroje
  • Carbohydrate Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 391
http://linked.open...iv/tvurceVysledku
  • Pazourek, Jiří
http://linked.open...ain/vavai/riv/wos
  • 000335902600011
issn
  • 0008-6215
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.carres.2014.03.010
http://localhost/t...ganizacniJednotka
  • 16370
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