About: Identification of carbohydrate isomers in flavonoid glycosides after hydrolysis by hydrophilic interaction chromatography

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A method for carbohydrate isomers (saccharide units) identification in flavonoid glycosides after hydrolysis by an HPLC system with two detectors (diode array UV-VIS detector and evaporative light scattering detector) was set up in this work. Experimental procedure was optimized with two types of model glycosides, namely rutin and hesperidin. The model glycosides were hydrolyzed to saccharide units and aglycone parts; the aglycone was characterized by its UV-VIS spectrum and the saccharide unit was identified by its retention time and elution profile (anomeric signals). Acidic and enzymatic hydrolyses were compared in the first step of the method; in acidic hydrolysis, trifluoracetic acid was used, while glucosidase, galactosidase, and hesperidinase were used in enzymatic hydrolysis. A complete enzymatic hydrolysis was achieved with hesperidin and neohesperidin, but not with 3-O-glycosides. The method was applied for the identification of a glycone from a glycoside isolated from Polygonum lapathifolium (Polygonaceae sp.). A method for carbohydrate isomers (saccharide units) identification in flavonoid glycosides after hydrolysis by an HPLC system with two detectors (diode array UV-VIS detector and evaporative light scattering detector) was set up in this work. Experimental procedure was optimized with two types of model glycosides, namely rutin and hesperidin. The model glycosides were hydrolyzed to saccharide units and aglycone parts; the aglycone was characterized by its UV-VIS spectrum and the saccharide unit was identified by its retention time and elution profile (anomeric signals). Acidic and enzymatic hydrolyses were compared in the first step of the method; in acidic hydrolysis, trifluoracetic acid was used, while glucosidase, galactosidase, and hesperidinase were used in enzymatic hydrolysis. A complete enzymatic hydrolysis was achieved with hesperidin and neohesperidin, but not with 3-O-glycosides. The method was applied for the identification of a glycone from a glycoside isolated from Polygonum lapathifolium (Polygonaceae sp.). (en)
Title	Identification of carbohydrate isomers in flavonoid glycosides after hydrolysis by hydrophilic interaction chromatography Identification of carbohydrate isomers in flavonoid glycosides after hydrolysis by hydrophilic interaction chromatography (en)
skos:prefLabel	Identification of carbohydrate isomers in flavonoid glycosides after hydrolysis by hydrophilic interaction chromatography Identification of carbohydrate isomers in flavonoid glycosides after hydrolysis by hydrophilic interaction chromatography (en)
skos:notation	RIV/62157124:16370/13:43872332!RIV14-MSM-16370___
http://linked.open...avai/predkladatel	Farmaceutická fakulta
http://linked.open...avai/riv/aktivita	V
http://linked.open...avai/riv/aktivity	V
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Pazourek, Jiří Špačková, Věra
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Farmaceutická fakulta
http://linked.open...dnocenehoVysledku	78663
http://linked.open...ai/riv/idVysledku	RIV/62157124:16370/13:43872332
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	HILIC; enzymatic hydrolysis; acidic hydrolysis; identification of isomers; glycosides (en)
http://linked.open.../riv/klicoveSlovo	glycosides acidic hydrolysis identification of isomers enzymatic hydrolysis HILIC
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[C97C3D7AF12E]
http://linked.open...i/riv/nazevZdroje	Chemical Papers - Chemické Zvesti
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	67
http://linked.open...iv/tvurceVysledku	Pazourek, Jiří Špačková, Věra
http://linked.open...ain/vavai/riv/wos	000313294700001
issn	0366-6352
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.2478/s11696-012-0302-8
http://localhost/t...ganizacniJednotka	16370

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