About: Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Oxidative stress plays a key role in the pathophysiology of many diseases. Hydroxyl radical is the oxidative species most commonly causing damage to cells. The aim of this work was to optimize the method for antioxidant activity determination on a model lipophilic geranylated flavanone, diplacone. This method uses protection of plasmid DNA from oxidation by a hydroxyl radical generated by the Fenton reaction involving oxidation of metal ions using H2O2 and ascorbate. The method was optimized for lipophilic compounds using several solvents and co-solvents. It was found that (2-hydroxypropyl)-beta-cyclodextrin (0.1 mass % aq. sol.) is the best co-solvent for our model lipophilic compound to measure the antioxidant activity by the method presented. Other solvents, namely dimethyl sulfoxide, Cremophor ELA (R) (0.1 mass % aq. sol.), ethanol, and methanol, were not suitable for the determination of the antioxidant activity by the method described. Tween 80 (0.1 mass % aq. sol.) and a mixture of 10 vol. % ethanol and 9 mass % bovine serum albumin (aq. sol.) significantly decreased the antioxidant activity of the model lipophilic compound and thus were not suitable for this method. Oxidative stress plays a key role in the pathophysiology of many diseases. Hydroxyl radical is the oxidative species most commonly causing damage to cells. The aim of this work was to optimize the method for antioxidant activity determination on a model lipophilic geranylated flavanone, diplacone. This method uses protection of plasmid DNA from oxidation by a hydroxyl radical generated by the Fenton reaction involving oxidation of metal ions using H2O2 and ascorbate. The method was optimized for lipophilic compounds using several solvents and co-solvents. It was found that (2-hydroxypropyl)-beta-cyclodextrin (0.1 mass % aq. sol.) is the best co-solvent for our model lipophilic compound to measure the antioxidant activity by the method presented. Other solvents, namely dimethyl sulfoxide, Cremophor ELA (R) (0.1 mass % aq. sol.), ethanol, and methanol, were not suitable for the determination of the antioxidant activity by the method described. Tween 80 (0.1 mass % aq. sol.) and a mixture of 10 vol. % ethanol and 9 mass % bovine serum albumin (aq. sol.) significantly decreased the antioxidant activity of the model lipophilic compound and thus were not suitable for this method. (en)
Title	Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization (en)
skos:prefLabel	Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization Determination of antioxidant activity using oxidative damage to plasmid DNA: pursuit of solvent optimization (en)
skos:notation	RIV/62157124:16370/13:43871809!RIV14-MSM-16370___
http://linked.open...avai/predkladatel	Farmaceutická fakulta
http://linked.open...avai/riv/aktivita	S
http://linked.open...avai/riv/aktivity	S
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Šmejkal, Karel Žemlička, Milan Hošek, Jan Treml, Jakub
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Farmaceutická fakulta
http://linked.open...dnocenehoVysledku	68851
http://linked.open...ai/riv/idVysledku	RIV/62157124:16370/13:43871809
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	diplacone; solubility; Fenton reaction; plasmid DNA; antioxidant aktivity (en)
http://linked.open.../riv/klicoveSlovo	Fenton reaction plasmid DNA antioxidant aktivity diplacone solubility
http://linked.open...odStatuVydavatele	SK - Slovenská republika
http://linked.open...ontrolniKodProRIV	[B56F0A38A0CD]
http://linked.open...i/riv/nazevZdroje	Chemical Papers - Chemické Zvesti
http://linked.open...in/vavai/riv/obor	FR
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	67
http://linked.open...iv/tvurceVysledku	Hošek, Jan Šmejkal, Karel Žemlička, Milan Treml, Jakub
http://linked.open...ain/vavai/riv/wos	000314882900002
issn	0366-6352
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.2478/s11696-013-0334-8
http://localhost/t...ganizacniJednotka	16370

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