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Description
| - An assay for the measurement of antiradical activity profiles of free radical scavengers (sometimes called antioxidants) in plant extracts was optimised on a modular instrument to obtain rapid and precise results. The first step of a run was separation by RPHPLC recorded with a direct absorbance detector, followed by a post-column reaction with a radical form of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS(+)). Then, the effect of colour bleaching of ABTS(+) was measured on a second absorbance detector (indirect detection). A comparison of the two signals distinguished the analytes with antiradical activity. Improvements in technical features, namely the minimisation of reaction volume and the use of precise high-pressure pumps enabled both the detector signals to be precisely matched without significant loss of separation efficiency, even in complex chromatograms.
- An assay for the measurement of antiradical activity profiles of free radical scavengers (sometimes called antioxidants) in plant extracts was optimised on a modular instrument to obtain rapid and precise results. The first step of a run was separation by RPHPLC recorded with a direct absorbance detector, followed by a post-column reaction with a radical form of 2,2'-azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS(+)). Then, the effect of colour bleaching of ABTS(+) was measured on a second absorbance detector (indirect detection). A comparison of the two signals distinguished the analytes with antiradical activity. Improvements in technical features, namely the minimisation of reaction volume and the use of precise high-pressure pumps enabled both the detector signals to be precisely matched without significant loss of separation efficiency, even in complex chromatograms. (en)
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Title
| - An optimised method for the rapid measurement and calculation of radical scavenger profiles in plant extracts by HPLC
- An optimised method for the rapid measurement and calculation of radical scavenger profiles in plant extracts by HPLC (en)
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skos:prefLabel
| - An optimised method for the rapid measurement and calculation of radical scavenger profiles in plant extracts by HPLC
- An optimised method for the rapid measurement and calculation of radical scavenger profiles in plant extracts by HPLC (en)
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skos:notation
| - RIV/62157124:16370/11:43870892!RIV12-MSM-16370___
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http://linked.open...avai/predkladatel
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16370/11:43870892
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Antioxidants; Calculation of radical scavenger profile; Plant extracts; ABTS assay; HPLC (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Pazourek, Jiří
- Václavík, Jiří
- Žemlička, Milan
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http://linked.open...ain/vavai/riv/wos
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issn
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number of pages
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http://bibframe.org/vocab/doi
| - 10.1016/j.foodchem.2010.09.031
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http://localhost/t...ganizacniJednotka
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is http://linked.open...avai/riv/vysledek
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