About: Performance of phased rotation, conformation and translation function: accurate protein model building with tripeptidic and terapeptidic fragments     Goto   Sponge   NotDistinct   Permalink

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  • The automatic building of protein structures with tripeptidic and tetrapeptidic fragments was investigated. The oligopeptidic conformers were positioned in the electron-density map by a phased rotation, conformation and translation function and refined by a real-space refinement. The number of successfully located fragments lies within the interval 75 ? 95 % depending on the resolution and phase quality. The overlaps of partially located fragments were analyzed. The correctly positioned fragments were connected into chains. Chains formed in this way are extended directly into the electron density and a sequence was assigned. In the initial stage of the model building the number of located fragments was between 60 ? 95 % but this number could be increased by several cycles of reciprocal-space refinement and automatic model re-building. A nearly complete structure can be obtained on the condition that the resolution is reasonable. Computer graphics will only needed for a final check and small correction
  • The automatic building of protein structures with tripeptidic and tetrapeptidic fragments was investigated. The oligopeptidic conformers were positioned in the electron-density map by a phased rotation, conformation and translation function and refined by a real-space refinement. The number of successfully located fragments lies within the interval 75 ? 95 % depending on the resolution and phase quality. The overlaps of partially located fragments were analyzed. The correctly positioned fragments were connected into chains. Chains formed in this way are extended directly into the electron density and a sequence was assigned. In the initial stage of the model building the number of located fragments was between 60 ? 95 % but this number could be increased by several cycles of reciprocal-space refinement and automatic model re-building. A nearly complete structure can be obtained on the condition that the resolution is reasonable. Computer graphics will only needed for a final check and small correction (en)
Title
  • Performance of phased rotation, conformation and translation function: accurate protein model building with tripeptidic and terapeptidic fragments
  • Performance of phased rotation, conformation and translation function: accurate protein model building with tripeptidic and terapeptidic fragments (en)
skos:prefLabel
  • Performance of phased rotation, conformation and translation function: accurate protein model building with tripeptidic and terapeptidic fragments
  • Performance of phased rotation, conformation and translation function: accurate protein model building with tripeptidic and terapeptidic fragments (en)
skos:notation
  • RIV/62157124:16370/10:00002651!RIV11-MSM-16370___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6215712403)
http://linked.open...iv/cisloPeriodika
  • 9
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 278492
http://linked.open...ai/riv/idVysledku
  • RIV/62157124:16370/10:00002651
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Fragment; rotation function; translation function; conformation; protein model building; automatic structure (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [CE44D6C2740A]
http://linked.open...i/riv/nazevZdroje
  • Acta Crystallographica Section D
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 66
http://linked.open...iv/tvurceVysledku
  • Václavík, Jiří
  • Pavelčík, František
http://linked.open...ain/vavai/riv/wos
  • 000281635500007
http://linked.open...n/vavai/riv/zamer
issn
  • 0907-4449
number of pages
http://localhost/t...ganizacniJednotka
  • 16370
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