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  • This study is focused on the comparison and characterization of Escherichia coli (E. coli) isolates obtained from the animal products - meat (n=59), offal (n=20), raw cow's milk (n = 84). Resistance to antimicrobial agents and detection of selected resistance genes was performed. Using the disk diffusion method, resistance to minimum one antimicrobial agent was more often detected in isolates from offal (60,0 %) and meat (52,5 %) compare to the isolates from raw cow?s milk (20,2 %). Resistance to ampicillin was the most frequently detected in isolates from all commodities. In isolates from meat and offal, multidrug resistance was observed. Resistant genes responsible for the resistance to tetracycline tetA and tetB were detected in isolates from milk as well as in isolates from meat and offal. Gene tetC was detected only in two isolates from meat, tetG gene was not detected. Genes encoding resistance to beta-lactams (blaTEM) were detected only in isolates from meat and offal. In one isolate from meat blaCTXM gene responsible for the production of extended spectrum beta-lactamases (ESBLs) was detected. Results of our study showed that E. coli isolates of meat and offal origin are more frequently a reservoir of resistance genes than isolates from milk and so represent a bigger danger for consumers.
  • This study is focused on the comparison and characterization of Escherichia coli (E. coli) isolates obtained from the animal products - meat (n=59), offal (n=20), raw cow's milk (n = 84). Resistance to antimicrobial agents and detection of selected resistance genes was performed. Using the disk diffusion method, resistance to minimum one antimicrobial agent was more often detected in isolates from offal (60,0 %) and meat (52,5 %) compare to the isolates from raw cow?s milk (20,2 %). Resistance to ampicillin was the most frequently detected in isolates from all commodities. In isolates from meat and offal, multidrug resistance was observed. Resistant genes responsible for the resistance to tetracycline tetA and tetB were detected in isolates from milk as well as in isolates from meat and offal. Gene tetC was detected only in two isolates from meat, tetG gene was not detected. Genes encoding resistance to beta-lactams (blaTEM) were detected only in isolates from meat and offal. In one isolate from meat blaCTXM gene responsible for the production of extended spectrum beta-lactamases (ESBLs) was detected. Results of our study showed that E. coli isolates of meat and offal origin are more frequently a reservoir of resistance genes than isolates from milk and so represent a bigger danger for consumers. (en)
Title
  • Animal products as the source of resistant Escherichia coli
  • Animal products as the source of resistant Escherichia coli (en)
skos:prefLabel
  • Animal products as the source of resistant Escherichia coli
  • Animal products as the source of resistant Escherichia coli (en)
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  • RIV/62157124:16270/13:43872253!RIV14-MZE-16270___
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http://linked.open...avai/riv/aktivita
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  • P(QJ1230044), S
http://linked.open...vai/riv/dodaniDat
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  • 61270
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  • RIV/62157124:16270/13:43872253
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  • ESBL; resistence; E. coli; Enterobacteriaceae (en)
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  • [7EFCA86E1EF5]
http://linked.open...v/mistoKonaniAkce
  • Brno
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  • Brno
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  • Sborník příspěvků XV. konference mladých vědeckých pracovníků s mezinárodní účastí
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http://linked.open...iv/tvurceVysledku
  • Bogdanovičová, Kateřina
  • Karpíšková, Renata
  • Koláčková, Ivana
  • Skočková, Alena
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  • Veterinární a farmaceutická univerzita Brno
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  • 978-80-7305-650-6
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  • 16270
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