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| Description
| - The aim of this study is to compare pulsed field gel electrophoresis (PFGE - SmaI) subtypes and antimicrobial resistance of Campylobacter coli isolated from human patients and slaughtered pigs. The MIC values and presence of genes associated with resistance to ampicillin, ciprophloxacine, nalidixic acid, erythromycine, gentamycine, chloramphenicol and tetracycline for C. coli from humans (55) and pigs (188) were compared. Isolates from pigs were maily resistant to erythromycine (63%) with mutation A2075G in 23S rRNA gene also detected and tetracycline (48%) with tetO gene presence detected. Resistance to fluoroquinolones was low (27%). On the other hand isolates from human patients were highly resistant to nalidixic acid (49%), ciprofloxacine (46%) with Thr86Ile mutation in gyrA gene detected; erythromycin (24%) and tetracycline (6%). PFGE patterns were grouped into clusters of at least 80% genetic similarity. Among 55 human isolates 80% had unique genotypes; 20% represented five clusters of 2 to 3 isolates each. Among 188 pig isolates 36% had unique genotypes; 19% represented three clusters of 2 to 4 isolates each and 45% represented fourteen clusters of 5 to 11 isolates. Campylobacter population was genetically highly diverse and in all clusters only genotypes of one species (pig or human) were present. Study was supported by the project MSM621571240.
- The aim of this study is to compare pulsed field gel electrophoresis (PFGE - SmaI) subtypes and antimicrobial resistance of Campylobacter coli isolated from human patients and slaughtered pigs. The MIC values and presence of genes associated with resistance to ampicillin, ciprophloxacine, nalidixic acid, erythromycine, gentamycine, chloramphenicol and tetracycline for C. coli from humans (55) and pigs (188) were compared. Isolates from pigs were maily resistant to erythromycine (63%) with mutation A2075G in 23S rRNA gene also detected and tetracycline (48%) with tetO gene presence detected. Resistance to fluoroquinolones was low (27%). On the other hand isolates from human patients were highly resistant to nalidixic acid (49%), ciprofloxacine (46%) with Thr86Ile mutation in gyrA gene detected; erythromycin (24%) and tetracycline (6%). PFGE patterns were grouped into clusters of at least 80% genetic similarity. Among 55 human isolates 80% had unique genotypes; 20% represented five clusters of 2 to 3 isolates each. Among 188 pig isolates 36% had unique genotypes; 19% represented three clusters of 2 to 4 isolates each and 45% represented fourteen clusters of 5 to 11 isolates. Campylobacter population was genetically highly diverse and in all clusters only genotypes of one species (pig or human) were present. Study was supported by the project MSM621571240. (en)
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| Title
| - Comparison of Campylobacter coli isolated from pigs and humans in the Czech Republic
- Comparison of Campylobacter coli isolated from pigs and humans in the Czech Republic (en)
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| skos:prefLabel
| - Comparison of Campylobacter coli isolated from pigs and humans in the Czech Republic
- Comparison of Campylobacter coli isolated from pigs and humans in the Czech Republic (en)
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| skos:notation
| - RIV/62157124:16270/11:43870464!RIV12-MSM-16270___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16270/11:43870464
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - human; pigs; PFGE; antimicrobial resistance; Campylobacter (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...v/mistoKonaniAkce
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| http://linked.open...i/riv/mistoVydani
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| http://linked.open...i/riv/nazevZdroje
| - XVth ISAH Congress 2011 Proceedings Volume II
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Bořilová, Gabriela
- Nebola, Mojmír
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| http://linked.open...vavai/riv/typAkce
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| http://linked.open.../riv/zahajeniAkce
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| http://linked.open...n/vavai/riv/zamer
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| number of pages
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| http://purl.org/ne...btex#hasPublisher
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| https://schema.org/isbn
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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