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| rdf:type
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| Description
| - Clostridium perfringens patří mezi známé původce onemocnění lidí i zvířat. U drůbeže způsobuje nekrotickou enteritidu představující vážný ekonomický problém. V období od května 2005 do konce září 2006 bylo vyšetřeno celkem 609 vzorků obsahu slepých střev brojlerových kuřat z 23 farem s intenzivním způsobem výkrmu. Vzorky byly kultivovány na TCS agaru a krevním agaru, typické kolnie byly dále konfirmovány biochemicky. Stanovení toxinových genů bylo provedeno multiplexní polymerázovou reakcí (multiplex-PCR). Celkový počet pozitivních vzorků byl 112, což je asi 18%. Všechny izolované kmeny byly přiřazeny k typu A, u dvou z nich byl detekován gen pro β2 toxin.Clostridium perfringens patří mezi známé původce onemocnění lidí i zvířat. U drůbeže způsobuje nekrotickou enteritidu představující vážný ekonomický problém. V období od května 2005 do konce září 2006 bylo vyšetřeno celkem 609 vzorků obsahu slepých střev brojlerových kuřat z 23 farem s intenzivním způsobem výkrmu. Vzorky byly kultivovány na T (cs)
- Clostridium perfringens is a causative agent of human and animal foodborne diseases. It is known as a normal inhabitant of the intestinal tract of chickens as well as a potential pathogen causing necrotic enteritis. The aim of the present study was to detect the incidence of C. perfringens in healthy broiler chickens. From May 2005 to September 2006, 609 samples of caecal content from broilers from 23 intensive poultry farms were analyzed. The samples were cultivated on TSC and blood agar, typical colonies were identified and biochemically confirmed. The total number of positive samples was 112 (18.39%). The samples were processed by the multiplex polymerase chain reaction method (PCR) for toxin genotyping. The presence of alpha, beta, beta2 and enterotoxin gene was detected. All C. perfringens isolates were classified as type A, four isolates had the cpb2 gene. In conclusion the prevalence of C. perfringens positive farms is approximately 74% and the amount ranges about 104 cfu/g of caecal content.
- Clostridium perfringens is a causative agent of human and animal foodborne diseases. It is known as a normal inhabitant of the intestinal tract of chickens as well as a potential pathogen causing necrotic enteritis. The aim of the present study was to detect the incidence of C. perfringens in healthy broiler chickens. From May 2005 to September 2006, 609 samples of caecal content from broilers from 23 intensive poultry farms were analyzed. The samples were cultivated on TSC and blood agar, typical colonies were identified and biochemically confirmed. The total number of positive samples was 112 (18.39%). The samples were processed by the multiplex polymerase chain reaction method (PCR) for toxin genotyping. The presence of alpha, beta, beta2 and enterotoxin gene was detected. All C. perfringens isolates were classified as type A, four isolates had the cpb2 gene. In conclusion the prevalence of C. perfringens positive farms is approximately 74% and the amount ranges about 104 cfu/g of caecal content. (en)
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| Title
| - Incidence of Clostridium perfringens in broiler chickens in the Czech Republic
- Výskyt Clostridium perfringens u brojlerových kuřat v České republice (cs)
- Incidence of Clostridium perfringens in broiler chickens in the Czech Republic (en)
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| skos:prefLabel
| - Incidence of Clostridium perfringens in broiler chickens in the Czech Republic
- Výskyt Clostridium perfringens u brojlerových kuřat v České republice (cs)
- Incidence of Clostridium perfringens in broiler chickens in the Czech Republic (en)
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| skos:notation
| - RIV/62157124:16270/07:00001190!RIV08-MSM-16270___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16270/07:00001190
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Clostridium; necrotic enteritis; PCR; occurrence (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Nebola, Mojmír
- Steinhauserová, Iva
- Svobodová, Irena
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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