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| Description
| - Studie byla zaměřena na identifikaci patogenních kmenů Y. enterocolitica mikrobiologickými, biochemickými a PCR metodami. Vzorky byly odebírány z prasat, skotu, drůbeže a prostředí porážek. Pro izolaci Y. enterocolitica ze vzorků byly použity mikrobiologické metody s využitím kultivačních půd ITC, PSB, CIN. Patogenní kmeny Y. enterocolitica byly detekovány biochemickými a PCR metodami. Biochemickými metodami bylo identifikováno 138 patogenních kmenů Y. enterocolitica a PCR metodami 149 patogenních kmenů. PCR metody použité pro identifikaci patogenních kmenů jsou tedy více specifická než metody biochemické. Z celkového počtu odebraných vzorků (4617) bylo izolováno 149 patogenních kmenů Y. enterocolitica (120 kmenů z prasat, 19 z drůbeže, 8 ze skotu a 2 kmeny z prostředí porážek). (cs)
- In this study, pathogenic strains of Y. enterocolitica were identified by microbiological and PCR methods. The samples were collected from pigs, cattle, poultry, and slaughter houses. Three common techniques were used to isolate Y. enterocolitica from the samples - ITC, PSB, and direct on the CIN. Primers A1/A2, Y1/Y2, and rfbC 1/rfbC 2 were used for the specific detection of the pathogenic strains of Y. enterocolitica. Traditional microbiological methods were found to be insufficient for the specific identification of the Y. enterocolitica pathogen. In comparison with PCR which was able to detect 149 strains, the biochemical test could detect only 138 species. These results show that the use of biochemical methods of cultivation did not allow the identification of all Y. enterocolitica pathogens. In total, 149 strains of pathogenic Y. enterocolitica were examined of which 120 were from pigs, 19 from poultry, 8 were cattle strains, and 2 came from the environments of slaughterhouses.
- In this study, pathogenic strains of Y. enterocolitica were identified by microbiological and PCR methods. The samples were collected from pigs, cattle, poultry, and slaughter houses. Three common techniques were used to isolate Y. enterocolitica from the samples - ITC, PSB, and direct on the CIN. Primers A1/A2, Y1/Y2, and rfbC 1/rfbC 2 were used for the specific detection of the pathogenic strains of Y. enterocolitica. Traditional microbiological methods were found to be insufficient for the specific identification of the Y. enterocolitica pathogen. In comparison with PCR which was able to detect 149 strains, the biochemical test could detect only 138 species. These results show that the use of biochemical methods of cultivation did not allow the identification of all Y. enterocolitica pathogens. In total, 149 strains of pathogenic Y. enterocolitica were examined of which 120 were from pigs, 19 from poultry, 8 were cattle strains, and 2 came from the environments of slaughterhouses. (en)
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| Title
| - The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR
- The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR (en)
- Porovnání metod pro identifikaci patogenních sérotypů a biotypů Yersinia enterocolitica : Mikrobiologické metody a PCR (cs)
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| skos:prefLabel
| - The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR
- The comparison of the methods for the identification of pathogenic serotypes and biotypes of Yersinia enterocolitica: Microbiological methods and PCR (en)
- Porovnání metod pro identifikaci patogenních sérotypů a biotypů Yersinia enterocolitica : Mikrobiologické metody a PCR (cs)
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| skos:notation
| - RIV/62157124:16270/06:00000874!RIV07-MSM-16270___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16270/06:00000874
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Yersinia enterocolitica; pathogenic serotype; PCR detection; cultivation; DNA; identification; serotype; biotype (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Czech Journal of Food Science
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Steinhauserová, Iva
- Vázlerová, Miroslava
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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