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  • Three strains of Toxoplasma gondii isolated from rabbits and cat in the CESech Republic in 1994-1995 were maintained in a tissue cysts form in laboratory mice by injections of brain homogenate every 4-6 months. These strains were passaged 12 to 13 times before tachyzoites, from which DNA was isolated, were obtained. PCR/RFLP (restriction length polymorpism) (ROP1 gene/DdeI restriction endonuclease) and RFLP/DNA (RFLP with chromosomal (TGR1E probe/PstI and SalI restriction endonucleases) were used to characterize their genotyp. Genotype diferences were observed in the strain before and after increasing their pathogenicity.
  • Three strains of Toxoplasma gondii isolated from rabbits and cat in the CESech Republic in 1994-1995 were maintained in a tissue cysts form in laboratory mice by injections of brain homogenate every 4-6 months. These strains were passaged 12 to 13 times before tachyzoites, from which DNA was isolated, were obtained. PCR/RFLP (restriction length polymorpism) (ROP1 gene/DdeI restriction endonuclease) and RFLP/DNA (RFLP with chromosomal (TGR1E probe/PstI and SalI restriction endonucleases) were used to characterize their genotyp. Genotype diferences were observed in the strain before and after increasing their pathogenicity. (en)
Title
  • Genome changes in the Toxoplasma gondii strains during laboratory passages in mice
  • Genome changes in the Toxoplasma gondii strains during laboratory passages in mice (en)
skos:prefLabel
  • Genome changes in the Toxoplasma gondii strains during laboratory passages in mice
  • Genome changes in the Toxoplasma gondii strains during laboratory passages in mice (en)
skos:notation
  • RIV/62157124:16170/99:11!RIV/2000/MSM/161700
http://linked.open.../vavai/riv/strany
  • 25?
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 161700001)
http://linked.open...iv/cisloPeriodika
  • 2?
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
  • Literák, Ivan
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 741271
http://linked.open...ai/riv/idVysledku
  • RIV/62157124:16170/99:11
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Toxoplasma gondii (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [E1910A93EE64]
http://linked.open...i/riv/nazevZdroje
  • Acta Veterinaria (Brno)
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 1999
http://linked.open...iv/tvurceVysledku
  • Literák, Ivan
  • Rychlík, Ivan
http://linked.open...n/vavai/riv/zamer
issn
  • 0001-7213
number of pages
http://localhost/t...ganizacniJednotka
  • 16170
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