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| rdf:type
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| Description
| - Hoof diseases in dairy cows are an important research topic, where the most common problem is laminitis. Complete causes of this disease are still not fully understood. The critical period for the formation of laminitis is after the parturition when is increased demands for milk production and the food composition changes. This can cause a rumen acidosis where the rumen microflora also produces histamine, acting mainly on local microcirculation in hoofs. Many studies in vivo and in vitro have shown that keratinocytes are capable to produce matrix metalloproteinase -2 (MMP -2), which expression during laminitis is increased, and the cytokine IL- 1, which triggers a cascade of inflammation retroactively. Purpose of the project was induction cultures of bovine keratinocytes by various concentrations of histamine. Histamine was applied to the medium at a range of concentrations from the physiological to allergic reactions in plasma (0.15 to 1.0 mg / ml). At 24, 48 and 72 hours the concentration of MMP- 2 and IL -1 was measured in the medium by enzyme immunoassay (EIA). Comparison of levels of MMP-2 between tested cultures showed statistical significant interaction for concentration and time. For IL -1, this correlation was not statistically significant. Experiments have shown that histamine of various concentrations does not induce the production of MMP-2, or pre- inflammatory cytokine IL-1, rather their inhibition.
- Hoof diseases in dairy cows are an important research topic, where the most common problem is laminitis. Complete causes of this disease are still not fully understood. The critical period for the formation of laminitis is after the parturition when is increased demands for milk production and the food composition changes. This can cause a rumen acidosis where the rumen microflora also produces histamine, acting mainly on local microcirculation in hoofs. Many studies in vivo and in vitro have shown that keratinocytes are capable to produce matrix metalloproteinase -2 (MMP -2), which expression during laminitis is increased, and the cytokine IL- 1, which triggers a cascade of inflammation retroactively. Purpose of the project was induction cultures of bovine keratinocytes by various concentrations of histamine. Histamine was applied to the medium at a range of concentrations from the physiological to allergic reactions in plasma (0.15 to 1.0 mg / ml). At 24, 48 and 72 hours the concentration of MMP- 2 and IL -1 was measured in the medium by enzyme immunoassay (EIA). Comparison of levels of MMP-2 between tested cultures showed statistical significant interaction for concentration and time. For IL -1, this correlation was not statistically significant. Experiments have shown that histamine of various concentrations does not induce the production of MMP-2, or pre- inflammatory cytokine IL-1, rather their inhibition. (en)
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| Title
| - Induction of matrix metalloproteinase-2 and IL-1 in bovine keratinocytes by histamine.
- Induction of matrix metalloproteinase-2 and IL-1 in bovine keratinocytes by histamine. (en)
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| skos:prefLabel
| - Induction of matrix metalloproteinase-2 and IL-1 in bovine keratinocytes by histamine.
- Induction of matrix metalloproteinase-2 and IL-1 in bovine keratinocytes by histamine. (en)
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| skos:notation
| - RIV/62157124:16170/13:43872472!RIV14-MZE-16170___
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| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62157124:16170/13:43872472
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - histamine; keratinocytes (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...iv/tvurceVysledku
| - Šlosárková, Soňa
- Petrášová, Jana
- Korytárová, Natália
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| http://localhost/t...ganizacniJednotka
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