About: Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Quantification of viral infectious units is traditionally measured by methods based on forming plaques in semisolid media (PFU) or endpoint dilution of a virus-containing solution (TCID50), methods that are laborious, time-consuming and take on average 3-7 days to carry out. Quantitative real-time PCR is an established method to quantify nucleic acids at high accuracy and reproducibility, routinely used for virus detection and identification. In the present study, a procedure was developed using a twostep real-time PCR and the SYBR Green detection method to study whether there are correlations between TCID50/ml, PFU/ml and Ct values generated by real-time PCR enabling rapid and efficient calculation of titer equivalents when working with viruses in the research laboratory. In addition, an external standard with known concentrations was included using in vitro transcribed viral RNA, thus allowing the calculation of the amount of RNA copies needed for various applications (i.e. per plaque or T Quantification of viral infectious units is traditionally measured by methods based on forming plaques in semisolid media (PFU) or endpoint dilution of a virus-containing solution (TCID50), methods that are laborious, time-consuming and take on average 3-7 days to carry out. Quantitative real-time PCR is an established method to quantify nucleic acids at high accuracy and reproducibility, routinely used for virus detection and identification. In the present study, a procedure was developed using a twostep real-time PCR and the SYBR Green detection method to study whether there are correlations between TCID50/ml, PFU/ml and Ct values generated by real-time PCR enabling rapid and efficient calculation of titer equivalents when working with viruses in the research laboratory. In addition, an external standard with known concentrations was included using in vitro transcribed viral RNA, thus allowing the calculation of the amount of RNA copies needed for various applications (i.e. per plaque or T (en)
Title	Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR (en)
skos:prefLabel	Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR (en)
skos:notation	RIV/62157124:16170/09:00002511!RIV10-MSM-16170___
http://linked.open...avai/riv/aktivita	V Z
http://linked.open...avai/riv/aktivity	V, Z(SUJ200401)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Celer, Vladimír Rosenbergová, Kateřina Lány, Petr Molinková, Dobromila Pospíšil, Zdeněk
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Veterinární a farmaceutická univerzita Brno / Fakulta veterinárního lékařství
http://linked.open...dnocenehoVysledku	337827
http://linked.open...ai/riv/idVysledku	RIV/62157124:16170/09:00002511
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	TagMan probe (en)
http://linked.open.../riv/klicoveSlovo	TagMan probe
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[573D6FB215C4]
http://linked.open...i/riv/nazevZdroje	Veterinární medicína
http://linked.open...in/vavai/riv/obor	GJ
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	54
http://linked.open...iv/tvurceVysledku	Pospíšil, Zdeněk Celer, Vladimír Rosenbergová, Kateřina Lány, Petr Kubíček, Oldřich Molinková, Dobromila
http://linked.open...ain/vavai/riv/wos	000272392600007
http://linked.open...n/vavai/riv/zamer	Studium materiálových a lidských faktorů pro ochranu osob
issn	0375-8427
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	16170
is http://linked.open...avai/riv/vysledek of	Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR Quantification of avian influenza virus in tissues of mute swans using TaqMan real time RT-PCR

Faceted Search & Find service v1.16.116 as of Feb 22 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3239 as of Feb 22 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 68 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software