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  • Growth and viability of BY-2 cell suspension were compared during 5 days of culture in different media containing 2,4-D (2,4-dichlorophenoxyacetic acid) and/or its derivatives - 2,4-D-Asp (2,4-dichlorophenoxyacetyl-aspartic acid), Me-2,4-D (methylester of 2,4-D), 4-CPA (4-chlorophenoxyacetic acid). The highest viability of BY-2 cells was observed during culture in the medium with 2,4-D-Asp. The increased total cell number affected by 2,4-D-Asp was statistically significant on the second day of culture.For the study of the uptake of 2,4-D 14C-2,4-D was used. After subculture the cells accumulated more 14C-activity than in the stationary phase of growth. At the beginning of the culture cycle the suspensions with 2,4-D-Asp accumulated more 14C-activity than cell suspension under standard culture conditions.
  • Growth and viability of BY-2 cell suspension were compared during 5 days of culture in different media containing 2,4-D (2,4-dichlorophenoxyacetic acid) and/or its derivatives - 2,4-D-Asp (2,4-dichlorophenoxyacetyl-aspartic acid), Me-2,4-D (methylester of 2,4-D), 4-CPA (4-chlorophenoxyacetic acid). The highest viability of BY-2 cells was observed during culture in the medium with 2,4-D-Asp. The increased total cell number affected by 2,4-D-Asp was statistically significant on the second day of culture.For the study of the uptake of 2,4-D 14C-2,4-D was used. After subculture the cells accumulated more 14C-activity than in the stationary phase of growth. At the beginning of the culture cycle the suspensions with 2,4-D-Asp accumulated more 14C-activity than cell suspension under standard culture conditions. (en)
Title
  • Screening of 2,4-D and its derivatives oh growth of Tobacco BY-2 cell suspension
  • Screening of 2,4-D and its derivatives oh growth of Tobacco BY-2 cell suspension (en)
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  • Screening of 2,4-D and its derivatives oh growth of Tobacco BY-2 cell suspension
  • Screening of 2,4-D and its derivatives oh growth of Tobacco BY-2 cell suspension (en)
skos:notation
  • RIV/62156489:_____/03:21100013!RIV/2004/MSM/430004/N
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  • 53;53
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  • P(LN00A081)
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  • 626444
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  • RIV/62156489:_____/03:21100013
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  • 2; 4-D; Tobacco; BY-2 (en)
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  • [5B955AED205E]
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  • Viterbo, Italy
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  • Viterbo, Italy
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  • XXXIII Annual Meeting - Proceedings
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  • Havel, Ladislav
  • Procházka, Stanislav
  • Klemš, Marek
  • Smíšková, Alexandra
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  • Universita Tuscia
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