About: Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension     Goto   Sponge   NotDistinct   Permalink

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  • Malepszy and Solarek were the first who report somatic embryogenesis from cucumber cell suspension in 1986. The main Iimitation of cucumber embryogenesis is a high occurrence of abnormal embryos combined with a Iow ability and Branchard, 1993). For this work, we report on the induction of somatic embryos in cucumber hypocotyl suspension. The endogenous ABA Ievel in the cells are also discussed. One-week-old plants, derived from the surface sterilized seeds of cucumber (Cucumis sativus L., cv. Nora) grown on a solidified medium (MS, sucrose 5 9 r'), were used. Embryogenic cell suspension cultures from hypocotyl segments of cucumber have been initiated by 7-day exposure to MS medium supplemented with 3% sucrose, 2.2 mg r' 2,4-dichlorophenoxyacetic acid, and 100 mg r' casein hydrolyzate. The suspension cultures were maintained in the same liquid medium with 1.1 mg r1 2,4-D only on a horizontal shaker at 28°C, and 16/8 h photoperiod.
  • Malepszy and Solarek were the first who report somatic embryogenesis from cucumber cell suspension in 1986. The main Iimitation of cucumber embryogenesis is a high occurrence of abnormal embryos combined with a Iow ability and Branchard, 1993). For this work, we report on the induction of somatic embryos in cucumber hypocotyl suspension. The endogenous ABA Ievel in the cells are also discussed. One-week-old plants, derived from the surface sterilized seeds of cucumber (Cucumis sativus L., cv. Nora) grown on a solidified medium (MS, sucrose 5 9 r'), were used. Embryogenic cell suspension cultures from hypocotyl segments of cucumber have been initiated by 7-day exposure to MS medium supplemented with 3% sucrose, 2.2 mg r' 2,4-dichlorophenoxyacetic acid, and 100 mg r' casein hydrolyzate. The suspension cultures were maintained in the same liquid medium with 1.1 mg r1 2,4-D only on a horizontal shaker at 28°C, and 16/8 h photoperiod. (en)
Title
  • Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension
  • Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension (en)
skos:prefLabel
  • Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension
  • Somatic embryos development from cucumber (Cucumis sativus L.) cell suspension (en)
skos:notation
  • RIV/62156489:_____/01:21100014!RIV/2002/GA0/430002/N
http://linked.open.../vavai/riv/strany
  • 100;100
http://linked.open...avai/riv/aktivita
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  • P(GV206/96/K188)
http://linked.open...vai/riv/dodaniDat
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  • 696347
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  • RIV/62156489:_____/01:21100014
http://linked.open...riv/jazykVysledku
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  • cell suspension; Cucumber; in vi¨tro (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [141D58C565E7]
http://linked.open...v/mistoKonaniAkce
  • Nitra, Slovak Republic
http://linked.open...i/riv/mistoVydani
  • Nitra, Slovak Republic
http://linked.open...i/riv/nazevZdroje
  • Xth International Conference on Plan Embryology
http://linked.open...in/vavai/riv/obor
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http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Procházka, Stanislav
  • Klemš, Marek
  • Andrýsková, Lenka
  • Smíšková, Alexandra
http://linked.open...vavai/riv/typAkce
http://linked.open.../riv/zahajeniAkce
number of pages
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  • Neuveden
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