About: Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein

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About: Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://scialert.net/abstract/?doi=ijv.2014.243.252
Description	In this study, we reported sequencing and molecular analysis in detail of central part of 2BMP gene (approximately 270 nts) in case of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). For more complex analysis of genetic variability we added reference sequences of evaluated nepoviruses and also sequence of phylogenetically very similar Nepovirus GDefV. Range of sequence analysis showed 74.5-99.3% identity at the nucleotide level and 86.5-100% identity at the amino acid level for partial gene 2BMP among Czech, Italian, German, Swiss sequences and references of all isolates. In this study eleven isolates were sequenced consisting of 5 GFLV and 6 ArMV isolates. As expected, GFLV and ArMV sequences were distinguished based on sequence identities but differences were not significant. Surprisingly, GDefV sequence was almost identical with the majority of GFLV sequences, even more than some GFLV sequences between each other. Local complexity of nucleotide sequences was also evaluated and it was in average of 0.530 for GFLV and GDefV, for ArMV the average value was 0.533. This study provides detail insights for the genetic variability of GFLV, ArMV and GDefV in the central part of the 2BMP coding region. In this study, we reported sequencing and molecular analysis in detail of central part of 2BMP gene (approximately 270 nts) in case of Grapevine fanleaf virus (GFLV) and Arabis mosaic virus (ArMV). For more complex analysis of genetic variability we added reference sequences of evaluated nepoviruses and also sequence of phylogenetically very similar Nepovirus GDefV. Range of sequence analysis showed 74.5-99.3% identity at the nucleotide level and 86.5-100% identity at the amino acid level for partial gene 2BMP among Czech, Italian, German, Swiss sequences and references of all isolates. In this study eleven isolates were sequenced consisting of 5 GFLV and 6 ArMV isolates. As expected, GFLV and ArMV sequences were distinguished based on sequence identities but differences were not significant. Surprisingly, GDefV sequence was almost identical with the majority of GFLV sequences, even more than some GFLV sequences between each other. Local complexity of nucleotide sequences was also evaluated and it was in average of 0.530 for GFLV and GDefV, for ArMV the average value was 0.533. This study provides detail insights for the genetic variability of GFLV, ArMV and GDefV in the central part of the 2BMP coding region. (en)
Title	Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein (en)
skos:prefLabel	Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein Sequence Differences between Grapevine fanleaf virus and Arabis mosaic virus Isolates in RNA2 Encoding the Central Part of Movement Protein (en)
skos:notation	RIV/62156489:43510/14:00223083!RIV15-MZE-43510___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(QH91214)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Eichmeier, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Zahradnická fakulta (Lednice)
http://linked.open...dnocenehoVysledku	44532
http://linked.open...ai/riv/idVysledku	RIV/62156489:43510/14:00223083
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	sequencing; movement protein; RNA2 (en)
http://linked.open.../riv/klicoveSlovo	RNA2 movement protein sequencing
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[923D07E29AFF]
http://linked.open...i/riv/nazevZdroje	International journal of virology
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	1 (xsd:int)
http://linked.open...vavai/riv/projekt	New biotechnolgical approaches for nepovirus resitance creation in grapevine rootstock cultivars.
http://linked.open...UplatneniVysledku	2014
http://linked.open...v/svazekPeriodika	10
http://linked.open...iv/tvurceVysledku	Eichmeier, Aleš
issn	1816-4900
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.3923/ijv.2014.243.252
http://localhost/t...ganizacniJednotka	43510

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