About: Detection of Phytoplasma ESFY in Apricot Trees using Phloem and Petioles     Goto   Sponge   NotDistinct   Permalink

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Description
  • Fytoplazma ESFY (European stone fruit yellows phytoplasma), nově označovaná jako %22Candidatus Phytoplasma prunorum%22, je stále významným škodlivým činitelem v sadech jak meruňkových, tak broskvoňových. Způsoby rostlinolékařské diagnostiky fytoplazem jsou různé. Molekulárně genetickou diagnostikou s použitím metod PCR byl prokázán významný vliv termínu odběru vzorku na výsledek testování analyzovaného rostlinného materiálu.Současně byl prokázán rozdíl v přesnosti molekulární diagnostiky pro použití odlišných rostlinných pletiv (res- pektive lýka zdřevnatělého výhonu a u řapíku listu). Z výsledků je zřejmé, že DNA izolovaná z lýka poskytuje vyšší procento pozitivních výsledků testů na fytoplazmu ESFY než DNA izolovaná z listového řapíku. (cs)
  • ESFY phytoplasma (European stone fruit yellows phytoplasma) is nowadays one of the most important plant diseases, especially on apricots and peaches, and it belongs to the list of organisms for which quarantine is required in the Czech Republic. The aim of this study was to determine the best period for tissue extraction and the best technique for ESFY detection. It was also to investigate the possibility of isolating DNA for use in ESFY detection from the leaf-stalks of randomly chosen symptomaticand asymptomatic apricot trees. Results of the amplification of DNA extracted from leaf-stalk and phloem sampled from 2-year old woody shoots during the years 2003 and 2004 were statistically analysed and compared, and visible disease symptoms were simultaneously evaluated and compared to the results of molecular detection.
  • ESFY phytoplasma (European stone fruit yellows phytoplasma) is nowadays one of the most important plant diseases, especially on apricots and peaches, and it belongs to the list of organisms for which quarantine is required in the Czech Republic. The aim of this study was to determine the best period for tissue extraction and the best technique for ESFY detection. It was also to investigate the possibility of isolating DNA for use in ESFY detection from the leaf-stalks of randomly chosen symptomaticand asymptomatic apricot trees. Results of the amplification of DNA extracted from leaf-stalk and phloem sampled from 2-year old woody shoots during the years 2003 and 2004 were statistically analysed and compared, and visible disease symptoms were simultaneously evaluated and compared to the results of molecular detection. (en)
Title
  • Detection of Phytoplasma ESFY in Apricot Trees using Phloem and Petioles
  • Detekce fytoplazmy ESFY u meruněk s využitím lýka a řapíku (cs)
  • Detection of Phytoplasma ESFY in Apricot Trees using Phloem and Petioles (en)
skos:prefLabel
  • Detection of Phytoplasma ESFY in Apricot Trees using Phloem and Petioles
  • Detekce fytoplazmy ESFY u meruněk s využitím lýka a řapíku (cs)
  • Detection of Phytoplasma ESFY in Apricot Trees using Phloem and Petioles (en)
skos:notation
  • RIV/62156489:43510/05:00057363!RIV06-MZE-43510___
http://linked.open.../vavai/riv/strany
  • 132;140
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QC1359)
http://linked.open...iv/cisloPeriodika
  • 4
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 517487
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43510/05:00057363
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • PCR; apricot; phytoplasma ESFY; symptoms (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [86F3BB43CC5E]
http://linked.open...i/riv/nazevZdroje
  • Plant Protection Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 41
http://linked.open...iv/tvurceVysledku
  • Krška, Boris
  • Nečas, Tomáš
issn
  • 1212-2580
number of pages
http://localhost/t...ganizacniJednotka
  • 43510
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