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  • The aim of this work was to to investigate the suitability of various types of extractants for extraction of acid phosphomonoesterase from soil. Succinate-borate buff er of pH 4.8 or 0.1 M K2SO4 were the most effi cient to extract this enzyme compared to 0.1 M glutamic acid or 0.005 M salicylic acid. Extraction using 0.1 M glutamic acid gave signifi cantly (P<0.05) lowest extraction yield. The following extracts were obtained: clear K2SO4 and glutamic acid extracts, succinate-borate buff er extracts were of slight coloration, and in some cases, rose-colored extracts of salicylic acid. The results of this work are in accordance with low extraction yields of phosphomonoesterase reported in other studies.
  • The aim of this work was to to investigate the suitability of various types of extractants for extraction of acid phosphomonoesterase from soil. Succinate-borate buff er of pH 4.8 or 0.1 M K2SO4 were the most effi cient to extract this enzyme compared to 0.1 M glutamic acid or 0.005 M salicylic acid. Extraction using 0.1 M glutamic acid gave signifi cantly (P<0.05) lowest extraction yield. The following extracts were obtained: clear K2SO4 and glutamic acid extracts, succinate-borate buff er extracts were of slight coloration, and in some cases, rose-colored extracts of salicylic acid. The results of this work are in accordance with low extraction yields of phosphomonoesterase reported in other studies. (en)
Title
  • Extraction of acid phosphomonoesterase from soil: testing of various extractants.
  • Extraction of acid phosphomonoesterase from soil: testing of various extractants. (en)
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  • Extraction of acid phosphomonoesterase from soil: testing of various extractants.
  • Extraction of acid phosphomonoesterase from soil: testing of various extractants. (en)
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  • RIV/62156489:43410/11:00169235!RIV12-MSM-43410___
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  • Z(MSM6215648902)
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  • 3
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  • 199098
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  • RIV/62156489:43410/11:00169235
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  • salicylic acid; půda; glutamic acid; K2SO4; acid phosphomonoesterase (en)
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  • CZ - Česká republika
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  • [D722F45E2E6C]
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  • Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis
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  • 59
http://linked.open...iv/tvurceVysledku
  • Dundek, Peter
  • Formánek, Pavel
  • Holík, Ladislav
  • Janouš, Dalibor
  • Rejšek, Klement
  • Vranová, Valerie
http://linked.open...n/vavai/riv/zamer
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  • 1211-8516
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  • 43410
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