About: Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The method for supercritical fluid extraction (SFE) of amino acids was adapted and optimized for a dry leaves matrix. The matrix-dependent SFE method uses a mixture of MeOH:H2O:acetonitrile (10:10:1 v/v) as a modifier (0.5 ml in situ) at 70 °C and 40 MPa and no HCl is needed as an entrainer. The amino acids were quantified using high-performance liquid chromatography with fluorimetric detection (HPLC/FLD) after gradient elution on Zorbax Eclipse columns (4.6 x 75 mm) with aqueous Na2HPO4 buffer of pH 7.8 and ACN:MeOH:water as a mobile phase. In comparison with Soxhlet extraction, SFE gained higher recovery and selectivity, but it required longer extraction time (90 min) and it was more labor-intensive (clean-up step after the pre-concentration). Both methods should be used separately or as a combo according to the matrix, number of samples and levels of ballast compounds. The method for supercritical fluid extraction (SFE) of amino acids was adapted and optimized for a dry leaves matrix. The matrix-dependent SFE method uses a mixture of MeOH:H2O:acetonitrile (10:10:1 v/v) as a modifier (0.5 ml in situ) at 70 °C and 40 MPa and no HCl is needed as an entrainer. The amino acids were quantified using high-performance liquid chromatography with fluorimetric detection (HPLC/FLD) after gradient elution on Zorbax Eclipse columns (4.6 x 75 mm) with aqueous Na2HPO4 buffer of pH 7.8 and ACN:MeOH:water as a mobile phase. In comparison with Soxhlet extraction, SFE gained higher recovery and selectivity, but it required longer extraction time (90 min) and it was more labor-intensive (clean-up step after the pre-concentration). Both methods should be used separately or as a combo according to the matrix, number of samples and levels of ballast compounds. (en)
Title	Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection (en)
skos:prefLabel	Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection (en)
skos:notation	RIV/62156489:43410/08:00116481!RIV12-GA0-43410___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA526/05/0187), Z(MSM6215648902)
http://linked.open...iv/cisloPeriodika	8
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Lojková, Lea Kubáň, Vlastimil Hrdlička, Petr Buchta, Ivan Kula, Emanuel Klejdus, Bořivoj
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Lesnická a dřevařská fakulta
http://linked.open...dnocenehoVysledku	398353
http://linked.open...ai/riv/idVysledku	RIV/62156489:43410/08:00116481
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	birch leaves; chromatography; amino acids (en)
http://linked.open.../riv/klicoveSlovo	birch leaves chromatography amino acids
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[26A2360C5C1E]
http://linked.open...i/riv/nazevZdroje	Journal of Separation Science
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	The effect of site conditions and feed quality on the incidence and dispersion of eriophyid mites (Acari: Eriophyidae) of birch (Betula pendula Roth)
http://linked.open...UplatneniVysledku	2008
http://linked.open...v/svazekPeriodika	31
http://linked.open...iv/tvurceVysledku	Kula, Emanuel Kubáň, Vlastimil Klejdus, Bořivoj Hrdlička, Petr Lojková, Lea Buchta, Ivan
http://linked.open...n/vavai/riv/zamer	Forest and Wood: the support of functionally integrated forest management and use of wood as a renewable raw material
issn	1615-9306
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	43410
is http://linked.open...avai/riv/vysledek of	Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection Supercritical Fluid Extraction of Amino Acids from Birch (Betula pendula Roth) Leaves and their Liquid Chromatographic Determination with Fluorimetric Detection

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