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  • Direct organogenesis or callus formation of tobacco leaves was induced on a medium containing 0.8 mg.l-1 2,4-D, 0.5 mg.l-1 BAP and 13.2 mg.l-1 aspartic acid. Shoots or callus formation with globular structures depended on accumulation of 2,4-D in leaves and ethylene production. In long-term culture on medium with growth regulators the content of 2,4-D in leaves gradually increased, the highest 2,4-D content, similarly as the highest production of ethylene being detected after 2 weeks of culture. Globules excised from leaves emerged to shoots in culture where ethylene levels decreased by aeration, in contrary to culture without aeration in which callus was formed after excision. Short term culture (20 hours) of leaves on induction medium and subsequent culture on a basal medium resulted in direct organogenesis.
  • Direct organogenesis or callus formation of tobacco leaves was induced on a medium containing 0.8 mg.l-1 2,4-D, 0.5 mg.l-1 BAP and 13.2 mg.l-1 aspartic acid. Shoots or callus formation with globular structures depended on accumulation of 2,4-D in leaves and ethylene production. In long-term culture on medium with growth regulators the content of 2,4-D in leaves gradually increased, the highest 2,4-D content, similarly as the highest production of ethylene being detected after 2 weeks of culture. Globules excised from leaves emerged to shoots in culture where ethylene levels decreased by aeration, in contrary to culture without aeration in which callus was formed after excision. Short term culture (20 hours) of leaves on induction medium and subsequent culture on a basal medium resulted in direct organogenesis. (en)
Title
  • Interaction of 2,4-D and ethylene in organogenesis of tobacco leaves
  • Interaction of 2,4-D and ethylene in organogenesis of tobacco leaves (en)
skos:prefLabel
  • Interaction of 2,4-D and ethylene in organogenesis of tobacco leaves
  • Interaction of 2,4-D and ethylene in organogenesis of tobacco leaves (en)
skos:notation
  • RIV/62156489:43210/99:21100006!RIV/2000/MSM/432100
http://linked.open.../vavai/riv/strany
  • 51
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GV206/96/K188), Z(MSM 432100001)
http://linked.open...iv/cisloPeriodika
  • 42
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
  • Procházka, Stanislav
  • Reinöhl, Vilém
  • Fišerová, Helena
  • Klemš, Marek
  • Obdržálková, Lenka
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 742990
http://linked.open...ai/riv/idVysledku
  • RIV/62156489:43210/99:21100006
http://linked.open...riv/jazykVysledku
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [049C59C70E45]
http://linked.open...i/riv/nazevZdroje
  • Biologia plantarum
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 42
http://linked.open...iv/tvurceVysledku
  • Procházka, Stanislav
  • Reinöhl, Vilém
  • Fišerová, Helena
  • Klemš, Marek
  • Obdržálková, Lenka
http://linked.open...n/vavai/riv/zamer
issn
  • 0006-3134
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
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