About: Separation of lactoferrin from human saliva using monolithic disc

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow rate 4 mL min-1 and ionic strength of effluent as 2 uM NaCl. We estimated limit of detection of whole method, which was hyphenated to the Bradford's assay, down to 100 ng mL-1. The purity of the isolated fractions was verified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis and recovery of isolation was found to be 51 % using minimally processed sample of saliva. Further, we tested the optimized method on group of healthy volunteers (n = 7). We were able to distinguish between the healthy subjects and subject suffering from celiac disease, which reported at least 2.59 higher level of lactoferrin in comparison to healthy ones. The results were correlated with standard enzyme-linked immunosorbent assay (ELISA) kit with obtained correlation coefficient R2 = 0.8446. Analysis of lactoferrin in saliva by monolithic disc and subsequent offline photometric detection is faster and cheaper method compared to ELISA commercial kit. The total analysis of one sample takes \20 min. In this study, we optimized method for the isolation and detection of lactoferrin from human saliva using 3 mm short monolithic disc. We optimized the conditions for separation as flow rate 4 mL min-1 and ionic strength of effluent as 2 uM NaCl. We estimated limit of detection of whole method, which was hyphenated to the Bradford's assay, down to 100 ng mL-1. The purity of the isolated fractions was verified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis and recovery of isolation was found to be 51 % using minimally processed sample of saliva. Further, we tested the optimized method on group of healthy volunteers (n = 7). We were able to distinguish between the healthy subjects and subject suffering from celiac disease, which reported at least 2.59 higher level of lactoferrin in comparison to healthy ones. The results were correlated with standard enzyme-linked immunosorbent assay (ELISA) kit with obtained correlation coefficient R2 = 0.8446. Analysis of lactoferrin in saliva by monolithic disc and subsequent offline photometric detection is faster and cheaper method compared to ELISA commercial kit. The total analysis of one sample takes \20 min. (en)
Title	Separation of lactoferrin from human saliva using monolithic disc Separation of lactoferrin from human saliva using monolithic disc (en)
skos:prefLabel	Separation of lactoferrin from human saliva using monolithic disc Separation of lactoferrin from human saliva using monolithic disc (en)
skos:notation	RIV/62156489:43210/13:00213595!RIV14-GA0-43210___
http://linked.open...avai/predkladatel	Agronomická fakulta
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GAP102/11/1068)
http://linked.open...iv/cisloPeriodika	11-12
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Křížková, Soňa Adam, Vojtěch Kizek, René Skaličková, Sylvie Zítka, Ondřej
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	104733
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/13:00213595
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	lactoferrin; optimalization; separation (en)
http://linked.open.../riv/klicoveSlovo	separation lactoferrin optimalization
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[9C135FBE4383]
http://linked.open...i/riv/nazevZdroje	Chromatographia
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Nano-Electro-Bio-Tools for Biochemical and Molecularly-Biological Studies of Eukaryotic Cells (NanoBioTECell)
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	76
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Kizek, René Křížková, Soňa Skaličková, Sylvie Zítka, Ondřej Vlková, Monika
issn	0009-5893
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10337-013-2459-x
http://localhost/t...ganizacniJednotka	43210

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