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  • This paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using the real-time spectral analysis of the samples to increase its performance. A laser beam illuminated the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested.
  • This paper describes a new design of standard agarose gel electrophoresis procedure for nucleic acids analysis. The electrophoresis was improved by using the real-time spectral analysis of the samples to increase its performance. A laser beam illuminated the analysed sample at wavelength with the highest absorption of gel components. Thus, DNA band is detected well before it may exits a gel plate. A modified horizontal electrophoresis device was designed, developed and tested. (en)
Title
  • Improvement of electrophoresis performance by spectral analysis
  • Improvement of electrophoresis performance by spectral analysis (en)
skos:prefLabel
  • Improvement of electrophoresis performance by spectral analysis
  • Improvement of electrophoresis performance by spectral analysis (en)
skos:notation
  • RIV/62156489:43210/12:00198497!RIV13-GA0-43210___
http://linked.open...avai/predkladatel
http://linked.open...avai/riv/aktivita
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  • P(ED1.100/02/0123), P(GAP102/11/1068)
http://linked.open...iv/cisloPeriodika
  • 51
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  • 140923
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  • RIV/62156489:43210/12:00198497
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  • electrophoresis; nucleic acid; spectral analysis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NE - Nigerská republika
http://linked.open...ontrolniKodProRIV
  • [0928AC1323D2]
http://linked.open...i/riv/nazevZdroje
  • African Journal of Biotechnology
http://linked.open...in/vavai/riv/obor
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  • 11
http://linked.open...iv/tvurceVysledku
  • Kizek, René
  • Provazník, Ivo
  • Khayal, L A
issn
  • 1684-5315
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
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