| Attributes | Values |
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| rdf:type
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| Description
| - MicroRNAs (miRNAs) are small, phylogenetically conserved, noncoding RNAs that are estimated to regulate at least 30% of all protein encoding genes. Mature miRNAs are involved in post-transcriptional gene silencing. They arise from intergenic or intragenic (both exonic and intronic) genomic regions that are transcribed as long primary transcripts. In recent years, miRNAs have received attention as potential cancer diagnostic markers and therapeutic targets [1]. Deregulated miRNAs expressions were reported in various human cancers including prostate cancer. MiRNAs not only regulate various developmental and physiologic processes but also are involved in cancer development and can play roles as important biomarkers. The aim of this study was to determinate the expression levels of selected miRNA in the non-tumour PNT1A line in comparison with tumour lines 22Rv1, PC-3, and LNCaP. We used the TaqMan Gene Expression Assays and quantitative RT-PCR. We observed significantly higher (p<0.01) expression of miRNA 23a in all tumour lines compared to non-tumour PNT1A. In addition, significantly higher (p<0.01) expression of miRNA 224 in 22Rv1 line compared to others and significantly higher (p<0.01) expression of miRNA 375 in all tumour lines in comparison with non-tumour PNT1A was also determined.
- MicroRNAs (miRNAs) are small, phylogenetically conserved, noncoding RNAs that are estimated to regulate at least 30% of all protein encoding genes. Mature miRNAs are involved in post-transcriptional gene silencing. They arise from intergenic or intragenic (both exonic and intronic) genomic regions that are transcribed as long primary transcripts. In recent years, miRNAs have received attention as potential cancer diagnostic markers and therapeutic targets [1]. Deregulated miRNAs expressions were reported in various human cancers including prostate cancer. MiRNAs not only regulate various developmental and physiologic processes but also are involved in cancer development and can play roles as important biomarkers. The aim of this study was to determinate the expression levels of selected miRNA in the non-tumour PNT1A line in comparison with tumour lines 22Rv1, PC-3, and LNCaP. We used the TaqMan Gene Expression Assays and quantitative RT-PCR. We observed significantly higher (p<0.01) expression of miRNA 23a in all tumour lines compared to non-tumour PNT1A. In addition, significantly higher (p<0.01) expression of miRNA 224 in 22Rv1 line compared to others and significantly higher (p<0.01) expression of miRNA 375 in all tumour lines in comparison with non-tumour PNT1A was also determined. (en)
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| Title
| - Detection of selected miRNAs in prostate carcinoma
- Detection of selected miRNAs in prostate carcinoma (en)
|
| skos:prefLabel
| - Detection of selected miRNAs in prostate carcinoma
- Detection of selected miRNAs in prostate carcinoma (en)
|
| skos:notation
| - RIV/62156489:43210/12:00198409!RIV13-GA0-43210___
|
| http://linked.open...avai/predkladatel
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/12:00198409
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - detection; prostate carcinom; miRNA (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - International Journal of Molecular Medicine
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Adam, Vojtěch
- Babula, Petr
- Gumulec, Jaromír
- Hlavna, M.
- Kizek, René
- Masařík, Michal
- Sztalmachová, Markéta
- Raudenská, M.
- Tanhauserova, V.
- Hudcová, Kateřina
|
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
| is http://linked.open...avai/riv/vysledek
of | |
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