About: Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Ellipticine, an alkaloid isolated from Apocynaceae plants, exhibits an antitumor activity, which is exceptionally high against several specific types of tumors. Ellipticine is also interesting as an anticancer drug as it has limited side effects and lacks of hematological toxicity. Various methods to study intercalating activity of this drug have been developed. However, to our best knowledge, capillary electrophoresis (CE) as a technique combining high separation resolution with various detection options has never been used for these purposes. In this study, a novel separation method based on CE with laser-induced fluorescence detection has been developed for the determination of ellipticine and for the monitoring of ellipticine DNA interaction. Sodium acetate was used as a background electrolyte and LIF detection at lambdaex = 488 nm. The limit of detection for ellipticine was determined to be 5 x 10 8 M. Additionally, intercalation of ellipticine into the double-stranded DNA was investigated. Signal corresponding to ellipticine was decreasing and a new peak appeared and was growing. It can be concluded that CE-LIF is a method applicable to in vitro studies of ellipticine DNA complexes. Ellipticine, an alkaloid isolated from Apocynaceae plants, exhibits an antitumor activity, which is exceptionally high against several specific types of tumors. Ellipticine is also interesting as an anticancer drug as it has limited side effects and lacks of hematological toxicity. Various methods to study intercalating activity of this drug have been developed. However, to our best knowledge, capillary electrophoresis (CE) as a technique combining high separation resolution with various detection options has never been used for these purposes. In this study, a novel separation method based on CE with laser-induced fluorescence detection has been developed for the determination of ellipticine and for the monitoring of ellipticine DNA interaction. Sodium acetate was used as a background electrolyte and LIF detection at lambdaex = 488 nm. The limit of detection for ellipticine was determined to be 5 x 10 8 M. Additionally, intercalation of ellipticine into the double-stranded DNA was investigated. Signal corresponding to ellipticine was decreasing and a new peak appeared and was growing. It can be concluded that CE-LIF is a method applicable to in vitro studies of ellipticine DNA complexes. (en)
Title	Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection (en)
skos:prefLabel	Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection (en)
skos:notation	RIV/62156489:43210/12:00189242!RIV13-GA0-43210___
http://linked.open...avai/predkladatel	Agronomická fakulta
http://linked.open...avai/riv/aktivita	I P
http://linked.open...avai/riv/aktivity	I, P(ED1.1.00/02.0068), P(GAP301/10/0356)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Ryvolová, Markéta Eckschlager, Tomáš Adam, Vojtěch Kizek, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Mendelova univerzita v Brně / Agronomická fakulta
http://linked.open...dnocenehoVysledku	172149
http://linked.open...ai/riv/idVysledku	RIV/62156489:43210/12:00189242
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Ellipticine; DNA; interaction (en)
http://linked.open.../riv/klicoveSlovo	DNA interaction Ellipticine
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[EC71F1FFA569]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	Central european institute of technology Study of contribution of different DNA-damaging mechanisms to toxicity of cytostatics to human chemosensitive and chemoresistant neuroblastomas
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	33
http://linked.open...iv/tvurceVysledku	Adam, Vojtěch Eckschlager, Tomáš Kizek, René Ryvolová, Markéta Stiborová, Marie
issn	0173-0835
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	43210
is http://linked.open...avai/riv/vysledek of	Study of DNA-ellipticine interaction by capillary electrophoresis with laser-induced fluorescence detection

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software