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| Description
| - A new extraction technique based on the off line combination of pressurized liquid with solid phase extraction (PLE SPE) is described. The method was used for the extraction of bioactive phenolic acids (protocatechuic, p hydroxybenzoic, 2,3 dihydroxybenzoic, chlorogenic, vanillic, caffeic, p coumaric, salicylic acid), cinnamic acid and hydroxybenzaldehydes (p-hydroxybenzaldehyde, 3,4 dihydroxybenzaldehyde, vanillin) from in vitro culture of two freshwater algae (Anabaena doliolum and Spongiochloris spongiosa) and from food products of marine macroalgae Porphyra tenera (nori) and Undaria pinnatifida (wakame). For the identification and quantification of the compounds the molecular ions [M H] and specific fragments were analyzed by quadrupole mass spectrometry analyzer connected on-line with a reversed phase HPLC system. Our analysis showed that the freshwater algae and marine algal products contained submicrogram or microgram level of above mentioned phenols per gram of lyophilized sample. In addition, the total phenol content (Folin Ciocalteu assay) and antioxidant activity (TEAC assay, Trolox equivalent antioxidant capacity assay) of the PLE SPE extracts were determined and discussed.
- A new extraction technique based on the off line combination of pressurized liquid with solid phase extraction (PLE SPE) is described. The method was used for the extraction of bioactive phenolic acids (protocatechuic, p hydroxybenzoic, 2,3 dihydroxybenzoic, chlorogenic, vanillic, caffeic, p coumaric, salicylic acid), cinnamic acid and hydroxybenzaldehydes (p-hydroxybenzaldehyde, 3,4 dihydroxybenzaldehyde, vanillin) from in vitro culture of two freshwater algae (Anabaena doliolum and Spongiochloris spongiosa) and from food products of marine macroalgae Porphyra tenera (nori) and Undaria pinnatifida (wakame). For the identification and quantification of the compounds the molecular ions [M H] and specific fragments were analyzed by quadrupole mass spectrometry analyzer connected on-line with a reversed phase HPLC system. Our analysis showed that the freshwater algae and marine algal products contained submicrogram or microgram level of above mentioned phenols per gram of lyophilized sample. In addition, the total phenol content (Folin Ciocalteu assay) and antioxidant activity (TEAC assay, Trolox equivalent antioxidant capacity assay) of the PLE SPE extracts were determined and discussed. (en)
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| Title
| - Bioactive phenols from algae and their food products: application of accelerated- solvent and solid-phase extraction techniques
- Bioactive phenols from algae and their food products: application of accelerated- solvent and solid-phase extraction techniques (en)
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| skos:prefLabel
| - Bioactive phenols from algae and their food products: application of accelerated- solvent and solid-phase extraction techniques
- Bioactive phenols from algae and their food products: application of accelerated- solvent and solid-phase extraction techniques (en)
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| skos:notation
| - RIV/62156489:43210/10:00136091!RIV12-GA0-43210___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/62156489:43210/10:00136091
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - algae; phenols compounds; accelerated solvent extraction; Benzoic and cinnamic acid derivatives; Antioxidants (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Pharmaceutical and Biomedical Analysis
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kopecký, J.
- Vašíčková, Jana
- Vacek, Jan
- Klejdus, Bořivoj
- Stratil, Pavel
- Onofrejová, Lucia
- Mišurcová, L.
- Kráčmar, S.
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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| is http://linked.open...avai/riv/vysledek
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