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Description
  • We describe an inexpensive and reliable detector for measuring NO emitted in the gas phase from plants. The method relies on the use of a strong oxidizer to convert NO to NO2 and subsequent capture of NO2 by a Griess reagent trap. The set-up approaches the sensitivity for NO comparable to that of instruments based on chemiluminescence and photoacoustic detectors. We demonstrate the utility of our set-up by measuring NO produced by a variety of well-established plant sources. NO produced by nitrate reductase (NR) in tobacco leaves and barley aleurone was readily detected as was the production of NO from nitrite by the incubation medium of barley aleurone. Arabidopsis mutants that overproduce NO or lack NO-synthase (AtNOS1) also displayed the expected NO synthesis phenotype when assayed by our set-up. We could also measure NO production from elicitor-treated suspension cultured cells using this set-up. Further, we have focused on detection of NO by a widely used fluorescent probe 4-amino-5-methylamino-2
  • We describe an inexpensive and reliable detector for measuring NO emitted in the gas phase from plants. The method relies on the use of a strong oxidizer to convert NO to NO2 and subsequent capture of NO2 by a Griess reagent trap. The set-up approaches the sensitivity for NO comparable to that of instruments based on chemiluminescence and photoacoustic detectors. We demonstrate the utility of our set-up by measuring NO produced by a variety of well-established plant sources. NO produced by nitrate reductase (NR) in tobacco leaves and barley aleurone was readily detected as was the production of NO from nitrite by the incubation medium of barley aleurone. Arabidopsis mutants that overproduce NO or lack NO-synthase (AtNOS1) also displayed the expected NO synthesis phenotype when assayed by our set-up. We could also measure NO production from elicitor-treated suspension cultured cells using this set-up. Further, we have focused on detection of NO by a widely used fluorescent probe 4-amino-5-methylamino-2 (en)
  • Popisujeme nenáročný a spolehlivý detektor pro měření NO emitovaného rostlinami do plynné fáze. (cs)
Title
  • Measuring NO production by plant tissues and suspension cultured cells
  • Měření produkce NO rostlinnými pletivy a buněčnými suspenzními kulturami (cs)
  • Measuring NO production by plant tissues and suspension cultured cells (en)
skos:prefLabel
  • Measuring NO production by plant tissues and suspension cultured cells
  • Měření produkce NO rostlinnými pletivy a buněčnými suspenzními kulturami (cs)
  • Measuring NO production by plant tissues and suspension cultured cells (en)
skos:notation
  • RIV/62156489:43210/08:00117842!RIV08-MSM-43210___
http://linked.open.../vavai/riv/strany
  • 270;284
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  • P(1P05ME757)
http://linked.open...iv/cisloPeriodika
  • 2
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  • 378205
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  • RIV/62156489:43210/08:00117842
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  • nitric oxide; oxidizer column; Griess reagent (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [AC3D967B4AFF]
http://linked.open...i/riv/nazevZdroje
  • Molecular Plant
http://linked.open...in/vavai/riv/obor
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  • 1
http://linked.open...iv/tvurceVysledku
  • Reinöhl, Vilém
  • Víteček, Jan
  • Jones, Russell L.
issn
  • 1674-2052
number of pages
http://localhost/t...ganizacniJednotka
  • 43210
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